CAJ | 학술논문

目的：探讨当归红芪超滤物延缓老龄大鼠脑衰老的作用及其机制。方法选用3月龄大鼠12只作为青年组，选用24月龄大鼠36只随机分为老年组、当归红芪超滤物组、维生素E组。给予相应处理后检测各组学习记忆功能、脑指数测定，组织形态学观察，脑组织中超氧化物歧化酶（ SOD）活力，丙二醛（ MDA）含量测定及脑组织线粒体DNA（ mt-DNA）缺失的表达。结果与青年组大鼠相比：老年组大鼠学习能力下降，脑组织形态学存在差异，脑指数存在差异（P＜0.05），脑组织 SOD活力明显降低，MDA 含量明显升高，脑缺失 mt-DNA／内参 mt-DNA 比例明显增高（P＜0.05）；与老年组相比：用药组大鼠学习能力明显增加，脑组织形态学存在差异，脑指数无明显差异，脑组织中SOD 活力显著增高，脑组织中 MDA 含量显著降低，脑缺失mt-DNA／内参mt-DNA比例降低（ P＜0.05）。与维生素E组相比，用药组大鼠学习能力、脑指数、脑组织 SOD 活力、MDA含量、脑缺失mt-DNA／内参mt-DNA比例均无显著差异（ P＞0.05）。结论当归红芪超滤物可能通过清除自由基，防止mt-DNA片段丢失发挥延缓老龄大鼠脑组织衰老的作用，其功效与维生素E相当。
목적：탐토당귀홍기초려물연완노령대서뇌쇠로적작용급기궤제。방법선용3월령대서12지작위청년조，선용24월령대서36지수궤분위노년조、당귀홍기초려물조、유생소E조。급여상응처리후검측각조학습기억공능、뇌지수측정，조직형태학관찰，뇌조직중초양화물기화매（ SOD）활력，병이철（ MDA）함량측정급뇌조직선립체DNA（ mt-DNA）결실적표체。결과여청년조대서상비：노년조대서학습능력하강，뇌조직형태학존재차이，뇌지수존재차이（P＜0.05），뇌조직 SOD활력명현강저，MDA 함량명현승고，뇌결실 mt-DNA／내삼 mt-DNA 비례명현증고（P＜0.05）；여노년조상비：용약조대서학습능력명현증가，뇌조직형태학존재차이，뇌지수무명현차이，뇌조직중SOD 활력현저증고，뇌조직중 MDA 함량현저강저，뇌결실mt-DNA／내삼mt-DNA비례강저（ P＜0.05）。여유생소E조상비，용약조대서학습능력、뇌지수、뇌조직 SOD 활력、MDA함량、뇌결실mt-DNA／내삼mt-DNA비례균무현저차이（ P＞0.05）。결론당귀홍기초려물가능통과청제자유기，방지mt-DNA편단주실발휘연완노령대서뇌조직쇠로적작용，기공효여유생소E상당。
Objective To investigate the effect of ultra-filtration extract from Angelica sinensis and Hedysari Polybotrys ( UFE-AH) on slowing brain aging of old rats .Methods 12 healthy male Wistar rats of 3-months old were selected for the youth control group ,and 36 natural aging male Wistar rats of 24-months old were selected and randomly divided into three groups:the old control ,UFE-AH and Vitamin E (VE) groups.After above treatments,the learning and memory ability and the brain index of each group of Wistar rats were measured ,brain tissue(hippocampus and cortex) was observed by microscope,the activity of dismutase(SOD),contents of maloudialdehyde(MDA) and the expression of mitochondrial DNA ( mt-DNA) deletion in the brains were tested .Results The learning and memory ability of old rats de-creased compared with the youth rats , there were significant difference in the brain tissue and brain index between the old and the youth (P<0.05).The activity of SOD apparently decreased ,contents of MDA apparently increased ,and the expression of mt-DNA deletion in-creased in old rats(P<0.05).Rats treated with UFE-AH had high learning and memory ability compared with the normal old rats ,there were significant difference in the brain tissue ,and there was no difference in brain index .The activity of SOD apparently increased ,contents of MDA apparently decreased,and the expression of mt-DNA deletion decreased of rats treated with UFE-AH(P<0.05).Rats treated with UFE-AH had no significant differences with the rats treated with VE in above parameters .Conclusions The UFE-AH could slow brain ag-ing of old rats by eliminating free oxygen radicals and preventing from mt-DNA deletion,whose function is similar with the VE .