中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2015年
4期
484-486,490
,共4页
印迹法,蛋白质%巨噬细胞游走抑制因子/代谢/药理学%宫颈肿瘤/代谢/病理学/药物疗法%细胞转化,肿瘤/药物作用%上皮细胞/药物作用/病理学%间质细胞/药物作用/病理学
印跡法,蛋白質%巨噬細胞遊走抑製因子/代謝/藥理學%宮頸腫瘤/代謝/病理學/藥物療法%細胞轉化,腫瘤/藥物作用%上皮細胞/藥物作用/病理學%間質細胞/藥物作用/病理學
인적법,단백질%거서세포유주억제인자/대사/약이학%궁경종류/대사/병이학/약물요법%세포전화,종류/약물작용%상피세포/약물작용/병이학%간질세포/약물작용/병이학
Blotting,Western%Macrophage migration-inhibitory factors/ME/PD%Uterine cervical neoplasms/ME/PA/DT%Cell transformation,neoplastic/DE%Epithelial cells/DE/PA%Stromal cells/DE/PA
目的 研究过表达巨噬细胞移动抑制因子(MIF)对宫颈癌SiHa细胞上皮间质转化(EMT)的影响作用.方法 通过构建重组真核表达载体构建pEGFP-N1-MIF,转染宫颈癌SiHa细胞(SiHa-pEGFP-N1-MIF组),同时设空载体转染组(SiHa-pEGFP-N1组)和空白对照组(SiHa细胞组),采用蛋白质印迹法(western blot W.B)检测SiHa细胞中MIF蛋白表达的变化,并检测转染前后SiHa细胞中EMT相关指标E-cadherin和Vimentin的表达变化.结果 pEGFP-N1-MIF转染SiHa细胞后MIF蛋白的表达量增加,高于对照组,差异有统计学意义(P＜0.05);且过表达MIF基因后,与对照组相比,pEGFP-N1-MIF组SiHa细胞上皮标志物E-cadherin的蛋白表达水平下调(P＜0.05),间叶标志物Vimentin的蛋白表达水平显著上调(P＜0.05).结论 过表达MIF基因可促进宫颈癌SiHa细胞发生上皮-间质转化的能力.
目的 研究過錶達巨噬細胞移動抑製因子(MIF)對宮頸癌SiHa細胞上皮間質轉化(EMT)的影響作用.方法 通過構建重組真覈錶達載體構建pEGFP-N1-MIF,轉染宮頸癌SiHa細胞(SiHa-pEGFP-N1-MIF組),同時設空載體轉染組(SiHa-pEGFP-N1組)和空白對照組(SiHa細胞組),採用蛋白質印跡法(western blot W.B)檢測SiHa細胞中MIF蛋白錶達的變化,併檢測轉染前後SiHa細胞中EMT相關指標E-cadherin和Vimentin的錶達變化.結果 pEGFP-N1-MIF轉染SiHa細胞後MIF蛋白的錶達量增加,高于對照組,差異有統計學意義(P＜0.05);且過錶達MIF基因後,與對照組相比,pEGFP-N1-MIF組SiHa細胞上皮標誌物E-cadherin的蛋白錶達水平下調(P＜0.05),間葉標誌物Vimentin的蛋白錶達水平顯著上調(P＜0.05).結論 過錶達MIF基因可促進宮頸癌SiHa細胞髮生上皮-間質轉化的能力.
목적 연구과표체거서세포이동억제인자(MIF)대궁경암SiHa세포상피간질전화(EMT)적영향작용.방법 통과구건중조진핵표체재체구건pEGFP-N1-MIF,전염궁경암SiHa세포(SiHa-pEGFP-N1-MIF조),동시설공재체전염조(SiHa-pEGFP-N1조)화공백대조조(SiHa세포조),채용단백질인적법(western blot W.B)검측SiHa세포중MIF단백표체적변화,병검측전염전후SiHa세포중EMT상관지표E-cadherin화Vimentin적표체변화.결과 pEGFP-N1-MIF전염SiHa세포후MIF단백적표체량증가,고우대조조,차이유통계학의의(P＜0.05);차과표체MIF기인후,여대조조상비,pEGFP-N1-MIF조SiHa세포상피표지물E-cadherin적단백표체수평하조(P＜0.05),간협표지물Vimentin적단백표체수평현저상조(P＜0.05).결론 과표체MIF기인가촉진궁경암SiHa세포발생상피-간질전화적능력.
Objective To investigate the effect of over-expressed macrophage migration inhibitory factor (MIF) on epithelial-mesenchymal transition (EMT) in human cervical carcinoma SiHa cells.Methods Recombinant eukaryotic expression plasmid liposome enhanced transfection of green fluorescent protein gene (pEGFP-N1)-MIF was constructed and then transfected into human cervical cancer SiHa cells.Experimental cells were classified into three groups (SiHa-pEGFP-N1-MIF,SiHa-pEGFP-N1,and SiHa).Western blot was used to detect the expression of MIF protein,and the expressions of EMT-related markers such as E-cadherin and vimentin in SiHa cells were determined before and after transfection.Results The eukaryotic expression vector pEGFP-N1-MIF significantly increased the expression of MIF protein in SiHa cells (P ＜ 0.05),and after overexpression of MIF gene in SiHa cells,the expression of E-cadherin protein in SiHa-pEGFP-N1-MIF group was significantly lower than that in control groups (P ＜0.05),while the expression of vimentin in SiHa-pEGFP-N1-MIF group was significantly higher than that in control groups (P ＜ 0.05).Conclusions Overexpression of MIF in cervical cancer SiHa cells can promote the EMT occurrence.
