CAJ | 학술논문

目的应用实时荧光PCR技术检测不同剂量60Co γ射线照射后不同时间永生化淋巴细胞系AHH-1中S100A4基因的表达变化情况,探讨其基因表达变化的剂量和时间效应关系.方法永生化淋巴细胞系AHH-1接受0、1、3、5、8、10、15、18 Gy60Coγ射线照射后4、8、12、24、48和72 h,利用反转录聚合酶链反应(PCR)以及实时荧光PCR技术检测细胞S100A4基因表达水平,分析各时间点其基因的相对表达水平与不同照射剂量之间的关系.结果照射后各时间点,一定剂量范围内,AHH-1中S100 A4基因的表达水平上调,与照射剂量存在着一定的剂量-效应关系(R2 =0.79～0.93,P＜0.05);在一定时间范围内,AHH-1中S100A4基因表达水平的上调受照射后时间的影响(F=8.91,P＜0.01).结论在一定剂量范围内,辐射诱导的S100A4基因表达在转录水平的变化检测便捷,具有较好的剂量-效应关系,初步具备作为生物剂量计的基本条件.
목적 응용실시형광PCR기술검측불동제량60Co γ사선조사후불동시간영생화림파세포계AHH-1중S100A4기인적표체변화정황,탐토기기인표체변화적제량화시간효응관계.방법 영생화림파세포계AHH-1접수0、1、3、5、8、10、15、18 Gy60Coγ사선조사후4、8、12、24、48화72 h,이용반전록취합매련반응(PCR)이급실시형광PCR기술검측세포S100A4기인표체수평,분석각시간점기기인적상대표체수평여불동조사제량지간적관계.결과 조사후각시간점,일정제량범위내,AHH-1중S100 A4기인적표체수평상조,여조사제량존재착일정적제량-효응관계(R2 =0.79～0.93,P＜0.05);재일정시간범위내,AHH-1중S100A4기인표체수평적상조수조사후시간적영향(F=8.91,P＜0.01).결론 재일정제량범위내,복사유도적S100A4기인표체재전록수평적변화검측편첩,구유교호적제량-효응관계,초보구비작위생물제량계적기본조건.
Objective To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation.Methods AHH-1 cells was exposed to different doses(0,1,3,5,8,10,15 and 18 Gy)of 60Co γ-rays,and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4,8,12,24,48 and 72 h after irradiation.Results Within the range of applied doses,the level of S100A4 gene expression was upregulated with a good dose-response (R2 =0.79-0.93,P ＜ 0.05) and had obvious difference at different time points (F =8.91,P ＜ 0.01).Conclusion S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation,and hence is applicable as a dosimeter.