CAJ | 학술논문

目的观察旱莲草对老年痴呆模型大鼠学习记忆及脑源性神经营养因子(brain derived neurotrophic factor,BDNF)表达的影响.方法选用健康成年SD大鼠,随机数字表法分为对照组、模型组、药物低、高剂量组,每组10只.采用皮下注射D-半乳糖联合双侧海马注射Aβ25-35构建痴呆模型大鼠,同时每天给予生理盐水或不同剂量的旱莲草水提物灌胃治疗8周.用药结束后,Morris水迷宫法观察各组大鼠的学习记忆能力;免疫组化染色法和Western Blot法观察各组大鼠大脑BDNF的表达.结果 Morris水迷宫实验结果显示:与对照组相比[(18.83±0.62)s,(41.98±3.96)％,(5.40±1.17)次],模型组大鼠的平均潜伏期明显延长,时间百分比和探索次数明显减少[(34.14±1.43)s,(33.71±3.82)％,(3.40±0.70)次;P＜0.01];与模型组相比,药物高剂量组大鼠的平均潜伏期明显缩短,时间百分比和探索次数明显增加(P＜0.01).免疫组化染色和Western Blot的结果均显示:模型组大鼠大脑BDNF表达明显降低(P＜0.01),药物治疗组BDNF的表达明显增加(P＜0.01),且随着剂量的增加,BDNF的表达也逐渐增多.结论旱莲草能够提高老年痴呆模型大鼠学习记忆能力,其作用机制可能与提高大脑BDNF的表达有关.
목적 관찰한련초대노년치태모형대서학습기억급뇌원성신경영양인자(brain derived neurotrophic factor,BDNF)표체적영향.방법 선용건강성년SD대서,수궤수자표법분위대조조、모형조、약물저、고제량조,매조10지.채용피하주사D-반유당연합쌍측해마주사Aβ25-35구건치태모형대서,동시매천급여생리염수혹불동제량적한련초수제물관위치료8주.용약결속후,Morris수미궁법관찰각조대서적학습기억능력;면역조화염색법화Western Blot법관찰각조대서대뇌BDNF적표체.결과 Morris수미궁실험결과현시:여대조조상비[(18.83±0.62)s,(41.98±3.96)％,(5.40±1.17)차],모형조대서적평균잠복기명현연장,시간백분비화탐색차수명현감소[(34.14±1.43)s,(33.71±3.82)％,(3.40±0.70)차;P＜0.01];여모형조상비,약물고제량조대서적평균잠복기명현축단,시간백분비화탐색차수명현증가(P＜0.01).면역조화염색화Western Blot적결과균현시:모형조대서대뇌BDNF표체명현강저(P＜0.01),약물치료조BDNF적표체명현증가(P＜0.01),차수착제량적증가,BDNF적표체야축점증다.결론 한련초능구제고노년치태모형대서학습기억능력,기작용궤제가능여제고대뇌BDNF적표체유관.
Objective To study the effect of Eclipta alba on learning and memory ability and brain derived neurotrophic factor(BDNF) in brain of Alzheimer's disease (AD) model rats.Methods Healthy adult SD rats were randomly divided into control group,model group,low dosage group and high dosage group.There were 10 rats in each group.The model of Alzheimer's disease was established with subcutaneous injection of D-galactose and microinjection Aβ25-35 on bilateral hippocampus.All rats were treated with saline solution or different dosage of Eclipta alba respectively lasting 8 weeks.Then the ability of learning and memory of AD rats was evaluated by the Morris water maze test.The levels of expression of BDNF in brain were determined by immunohistochemical staining method and Western Blot method.Results The Morris water maze test:the average escape latent period prolonged besides the percentage of the swimming time in the target quadrant from the total swimming time and the times across the platform((34.14± 1.43) s,(33.71±3.82) ％,(3.40±0.70) times) decreased significantly in model group compared with control group((18.83±0.62) s,(41.98±3.96) ％,(5.40± 1.17) times,P＜0.01).The average escape latent period shortened besides the percentage of the swimming time in the target quadrant from the total swimming time and the times across the platform increased significantly in high dosage group compared with the model group (P＜0.01).Immunohistochemical staining and Western blot:the level of expression of BDNF in brain in model group was prominently less than control group (P＜ 0.01).The BDNF level in drug treated groups was prominently higher than model group(P＜0.01).The expression of BDNF increased with the drug dosage increasing.Conclusion Eclipta alba can improve the learning and memory function of AD rats by enhance the expression of BDNF.