中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2015年
3期
201-203
,共3页
唐华兵%温晓红%黄会芝%刘惠
唐華兵%溫曉紅%黃會芝%劉惠
당화병%온효홍%황회지%류혜
性别%缺氧缺血%学习记忆%磁共振%超微结构
性彆%缺氧缺血%學習記憶%磁共振%超微結構
성별%결양결혈%학습기억%자공진%초미결구
Sex%Hypoxia-ischemia%Learning and memory%MRI%Ultrastructure
目的 探讨不同性别新生大鼠缺氧缺血性脑损伤(hypoxic ischemic brain damage,HIBD)后学习记忆功能及脑组织损伤的改变.方法 新生7d龄SD大鼠60只,采用随机数表法分为雄性对照组、雌性对照组、雄性HIBD组、雌性HIBD组,每组15只.HIBD模型采用Rice-Vannucci方法,HIBD后28 d应用Morris水迷宫实验来评估四组大鼠空间学习记忆功能;应用头颅MRI、电子显微镜评估脑组织损伤范围及损伤部位神经元突触超微结构的改变.结果 雄性对照组大鼠各项检测结果与雌性对照组比较无显著性差异,均P＞0.05;雄性HIBD组及雌性HIBD组大鼠与各自对照组比较,逃避潜伏期[(39.38± 11.40)svs(14.86±4.42)s、(30.14±7.18)s vs (18.41±5.03)s]延长,游泳距离[(15.31±1.77) cm vs (3.68±-1.50) cm、(13.18±1.79) cm vs (4.61±1.61) cm]延长,透射电镜观察突触间隙[(23.18±1.36) nm vs (19.24±1.51) nm、(21.40±1.71) nm vs (19.87±0.94) nm]增宽,均P＜0.05,且雄性HIBD组较雌性HIBD组改变更加明显,差异具有统计学意义(P＜0.05);雄性HIBD组大鼠头颅MRI上脑损伤容量较雌性HIBD组大鼠明显增大,差异具有统计学意义(P＜0.05).结论 雌性大鼠对HIBD的耐受能力和/或恢复能力可能较雄性大鼠强.
目的 探討不同性彆新生大鼠缺氧缺血性腦損傷(hypoxic ischemic brain damage,HIBD)後學習記憶功能及腦組織損傷的改變.方法 新生7d齡SD大鼠60隻,採用隨機數錶法分為雄性對照組、雌性對照組、雄性HIBD組、雌性HIBD組,每組15隻.HIBD模型採用Rice-Vannucci方法,HIBD後28 d應用Morris水迷宮實驗來評估四組大鼠空間學習記憶功能;應用頭顱MRI、電子顯微鏡評估腦組織損傷範圍及損傷部位神經元突觸超微結構的改變.結果 雄性對照組大鼠各項檢測結果與雌性對照組比較無顯著性差異,均P＞0.05;雄性HIBD組及雌性HIBD組大鼠與各自對照組比較,逃避潛伏期[(39.38± 11.40)svs(14.86±4.42)s、(30.14±7.18)s vs (18.41±5.03)s]延長,遊泳距離[(15.31±1.77) cm vs (3.68±-1.50) cm、(13.18±1.79) cm vs (4.61±1.61) cm]延長,透射電鏡觀察突觸間隙[(23.18±1.36) nm vs (19.24±1.51) nm、(21.40±1.71) nm vs (19.87±0.94) nm]增寬,均P＜0.05,且雄性HIBD組較雌性HIBD組改變更加明顯,差異具有統計學意義(P＜0.05);雄性HIBD組大鼠頭顱MRI上腦損傷容量較雌性HIBD組大鼠明顯增大,差異具有統計學意義(P＜0.05).結論 雌性大鼠對HIBD的耐受能力和/或恢複能力可能較雄性大鼠彊.
목적 탐토불동성별신생대서결양결혈성뇌손상(hypoxic ischemic brain damage,HIBD)후학습기억공능급뇌조직손상적개변.방법 신생7d령SD대서60지,채용수궤수표법분위웅성대조조、자성대조조、웅성HIBD조、자성HIBD조,매조15지.HIBD모형채용Rice-Vannucci방법,HIBD후28 d응용Morris수미궁실험래평고사조대서공간학습기억공능;응용두로MRI、전자현미경평고뇌조직손상범위급손상부위신경원돌촉초미결구적개변.결과 웅성대조조대서각항검측결과여자성대조조비교무현저성차이,균P＞0.05;웅성HIBD조급자성HIBD조대서여각자대조조비교,도피잠복기[(39.38± 11.40)svs(14.86±4.42)s、(30.14±7.18)s vs (18.41±5.03)s]연장,유영거리[(15.31±1.77) cm vs (3.68±-1.50) cm、(13.18±1.79) cm vs (4.61±1.61) cm]연장,투사전경관찰돌촉간극[(23.18±1.36) nm vs (19.24±1.51) nm、(21.40±1.71) nm vs (19.87±0.94) nm]증관,균P＜0.05,차웅성HIBD조교자성HIBD조개변경가명현,차이구유통계학의의(P＜0.05);웅성HIBD조대서두로MRI상뇌손상용량교자성HIBD조대서명현증대,차이구유통계학의의(P＜0.05).결론 자성대서대HIBD적내수능력화/혹회복능력가능교웅성대서강.
Objective To explore the effect of sex difference on learning and memory function and the brain tissue damage of neonatal SD rats with hypoxic-ischemic brain damage.Methods Sixty,7-day-old neonatal Sprague-Dawley rats,were divided into randomly:male control group (M group,n=15),female control group (F group,n=15),male hypoxic-ischemic brain damage group (MHIBD group,n=15) and female hypoxic-ischemic brain damage group (FHIBD group,n=15).A modified newborn rat model that had a combined hypoxic-ischemic brain damage as described by Rice-Vannucci was used.The Morris water maze was used to evaluate the ability of learning and memory.The brain MRI and transmission electron microscope(TEM) was used to evaluate the scope of brain tissue damage and the change of the synaptic ultrastructure.Results There were no differences in swimming distance,escape latency,synaptic cleft and damage brain volume between M group and F group(P＞0.05).Compare with M group and F group,MHIBD group and FHIBD group showed significant brain injury,longer escape latency ((39.38±11.40) s vs (14.86±4.42) s,(30.14±7.18)s vs (18.41±5.03) s),longer swimming distance ((15.31± 1.77) cm vs (3.68±1.50) cm,(13.18±1.79) cm vs (4.61±1.61) cm),and TEM showed the synaptic cleft was widened ((23.18± 1.36) nm vs (19.24± 1.51) nm,(21.40± 1.71) nm vs (19.87±0.94) nm),P＜0.05).MHIBD group was more seriously compromised than the FHIBD group(P＜0.05).The brain MRI showed the damage brain volume of MHIBD group were significantly larger than FHIBD group(P＜0.05).Conclusion After bypoxic ischemic brain damage,the tolerance of brain damage and / or post-injury recovery capabilities of female rats are stronger than males.
