CAJ | 학술논문

目的探讨天麻素(gastrodin,GAS)对脑缺血再灌注模型小鼠神经功能的保护作用及纹状体髓磷脂碱性蛋白(myelin basic protein,MBP)和高分子量神经丝蛋白(neurofilament high molecular weight,NF-H)水平的影响.方法将36只昆明小鼠按照随机数字表法分为对照组(Sham)、模型组(MCAO)和天麻素组(GAS).Sham组进行假手术(皮肤切开,分离颈动脉),MCAO组及GAS组通过右侧颈内动脉栓线术,建立大脑中动脉闭塞(middle cerebral artery occlusion,MCAO),Sham组和MCAO组术后即刻腹腔注射生理盐水,GAS组则注射天麻素(100 mg/kg),并在随后6d同一时间接受相同剂量的盐水或天麻素.术后第7天进行神经功能评分及氯化三苯基四氮唑(2,3,5-triphenyltetrazolium chloride,TTC)染色;应用免疫组化观察小鼠纹状体内MBP和NF-H的表达情况.结果 (1)模型组小鼠出现明显的神经功能障碍,天麻素组神经功能评分[(3.13±0.64)分]明显高于模型组[(1.38±0.52)分],差异具有统计学意义(P＜0.05).(2)TTC检测提示,模型组脑组织损伤侧出现明显梗死灶,给予天麻素干预后脑梗死容积明显减少,差异具有统计学意义(P＜0.05).(3)模型组MBP和NF-H染色的积分光密度(272 968.14± 1215.23,121 427.32±44.16)显著低于对照组(43 855.23±2434.16,275 321.00±926.15),天麻素组MBP和NF-H染色的积分光密度(321 531.20±2376.14,206 135.73±598.15)均高于模型组,差异具有统计学意义(P＜0.05).结论天麻素可以改善MCAO模型小鼠神经功能,并且对纹状体髓鞘和神经纤维具有保护作用.
목적 탐토천마소(gastrodin,GAS)대뇌결혈재관주모형소서신경공능적보호작용급문상체수린지감성단백(myelin basic protein,MBP)화고분자량신경사단백(neurofilament high molecular weight,NF-H)수평적영향.방법 장36지곤명소서안조수궤수자표법분위대조조(Sham)、모형조(MCAO)화천마소조(GAS).Sham조진행가수술(피부절개,분리경동맥),MCAO조급GAS조통과우측경내동맥전선술,건립대뇌중동맥폐새(middle cerebral artery occlusion,MCAO),Sham조화MCAO조술후즉각복강주사생리염수,GAS조칙주사천마소(100 mg/kg),병재수후6d동일시간접수상동제량적염수혹천마소.술후제7천진행신경공능평분급록화삼분기사담서(2,3,5-triphenyltetrazolium chloride,TTC)염색;응용면역조화관찰소서문상체내MBP화NF-H적표체정황.결과 (1)모형조소서출현명현적신경공능장애,천마소조신경공능평분[(3.13±0.64)분]명현고우모형조[(1.38±0.52)분],차이구유통계학의의(P＜0.05).(2)TTC검측제시,모형조뇌조직손상측출현명현경사조,급여천마소간예후뇌경사용적명현감소,차이구유통계학의의(P＜0.05).(3)모형조MBP화NF-H염색적적분광밀도(272 968.14± 1215.23,121 427.32±44.16)현저저우대조조(43 855.23±2434.16,275 321.00±926.15),천마소조MBP화NF-H염색적적분광밀도(321 531.20±2376.14,206 135.73±598.15)균고우모형조,차이구유통계학의의(P＜0.05).결론 천마소가이개선MCAO모형소서신경공능,병차대문상체수초화신경섬유구유보호작용.
Objective To investigate the effects of gastrodin on neural function and the expression of myelin basic protein (MBP) and neurofilament high molecular weight (NFH) in the striatum during cerebral ischemiareperfusion in mice.Methods 36 Kunming mice were randomly divided into sham group,MCAO group and gastrodin (GAS) group.The middle cerebral artery occlusion(MCAO) was established by artery embolization.The mice in sham group were received fake surgery and saline,and the mice in MCAO and GAS group were exposed to MCAO,and received saline and GAS (100 mg/(kg · d)) injection,respectively,immediately after the operation for 7 days.On the 8th day of operation,the neurological severity scores of the mice were observed and the volume ratio of the cerebral infarction was estimated by triphenyl tetrazolium chloride (TTC) staining.Immunohistochemistry was used to detect the MBP and NF-H in the striatum.Results (1) The mice in MCAO group showed significant neurologic deficient in comparison with sham group,and the neurological severity scores of gastrodin group(3.13±0.64) were significantly higher than that(1.38±0.52) of MCAO group (P＜0.05).(2) Results of TTC staining showed that the infarction volume was obviously larger in the injured cerebral tissue in MCAO group in comparison with sham group,and the volume ratio of the cerebral infarction significantly decreased after the intervention with GAS (P＜0.05).(3) The integral optical density of MBP(272968.14±1215.23) and NF-H(12 142.73±47.16) in MCAO group decreased as compared to that((43 855.23±2434.16),(275 321.00±926.15)) in sham group and GAS group((321 531.2±2376.14),(106 135.73±598.15)) (P＜0.05).Conclusion GAS can improve neural function of mice after middle cerebral artery occlusion,and it may play an important role in protecting myelin and nerve fibers of striatum.