CAJ | 학술논문

为优选庆阳市传统发酵豆瓣酱高产蛋白酶霉菌，以甘肃庆阳5县区20个自然发酵豆瓣酱为材料，采用察氏培养基分离纯化、平板透明圈法初筛，发酵液蛋白酶活力复筛，通过孢子数测定和菌体形态观察鉴定及18S rDNA 分子生物学鉴定.结果表明：所分离出的11株霉菌株中9株具有分解蛋白质的能力，其中 BDH5蛋白酶活性最高，达到了4351.68 U/g.对 BDH5初步鉴定及18S rDNA 鉴定，所优选的 BDH5菌种为米曲霉(Aspergillus tamarii ).
위우선경양시전통발효두판장고산단백매매균，이감숙경양5현구20개자연발효두판장위재료，채용찰씨배양기분리순화、평판투명권법초사，발효액단백매활력복사，통과포자수측정화균체형태관찰감정급18S rDNA 분자생물학감정.결과표명：소분리출적11주매균주중9주구유분해단백질적능력，기중 BDH5단백매활성최고，체도료4351.68 U/g.대 BDH5초보감정급18S rDNA 감정，소우선적 BDH5균충위미곡매(Aspergillus tamarii ).
To gain the high yield protease mold strains of traditional fermented soybean paste in Qingy-ang City,20 experimental samples were collected from self-made soybean paste by peasant household in 5 counties of Qingyang City,using plate streaking purified predominant strains,protease fermentation broth determination of rescreening,measured by the number of spores and bacterial morphology preliminary and identification of 18S rDNA molecular biology.The results showed that the total of 1 1 predominant strains were isolated,and 9 of them with resolve ability on protein.The protease activity of BDH5 was the highest, which reached to 4 35 1.68 U/g.By preliminary identification combined with 18S rDNA identification,the BDH5 was identified as Aspergillus tamarii.