CAJ | 학술논문

目的：通过观察cAMP反应元件结合蛋白（ CREB）基因表达探讨高脂膳食与运动对脑老化的影响。方法70只ICR小鼠按体重随机分为普通对照组、高脂对照组、运动对照组、普通脑老化组、脑老化＋高脂组、脑老化＋运动组及脑老化＋高脂＋运动组。造模10 w后，以 Morris水迷宫实验检测小鼠的学习记忆能力， RT-PCR检测小鼠大脑CREB基因的表达水平。结果与普通对照组相比，普通脑老化组、脑老化＋高脂组、脑老化＋高脂＋运动组及高脂对照组小鼠脑CREB基因表达水平明显降低（P＜0.05），运动对照组CREB 基因表达水平无明显变化。脑老化＋运动组小鼠脑CREB基因表达水平明显高于脑老化组（ P＜0.05）。结论脑老化小鼠 CREB基因表达水平降低。高脂膳食下调CREB 基因的表达，具有促脑老化效应。运动可以防止脑老化小鼠CREB基因的表达下降，具有抗脑老化效应。
목적：통과관찰cAMP반응원건결합단백（ CREB）기인표체탐토고지선식여운동대뇌노화적영향。방법70지ICR소서안체중수궤분위보통대조조、고지대조조、운동대조조、보통뇌노화조、뇌노화＋고지조、뇌노화＋운동조급뇌노화＋고지＋운동조。조모10 w후，이 Morris수미궁실험검측소서적학습기억능력， RT-PCR검측소서대뇌CREB기인적표체수평。결과여보통대조조상비，보통뇌노화조、뇌노화＋고지조、뇌노화＋고지＋운동조급고지대조조소서뇌CREB기인표체수평명현강저（P＜0.05），운동대조조CREB 기인표체수평무명현변화。뇌노화＋운동조소서뇌CREB기인표체수평명현고우뇌노화조（ P＜0.05）。결론뇌노화소서 CREB기인표체수평강저。고지선식하조CREB 기인적표체，구유촉뇌노화효응。운동가이방지뇌노화소서CREB기인적표체하강，구유항뇌노화효응。
Objective To explore the effects of high fat diet and exercise on the expression of cAMP response element binding pro-tein( CREB) gene in brain of mice.Methods Seventy mice were randomly divided into control , high fat diet, exercise, brain aging, brain aging +high fat diet, brain aging +exercise, brain aging +high fat diet +exercise groups.The experimental duration was 10 weeks.Morris water maze test was used to measure the learning and memory ability .Reverse transcription polymerase chain reaction ( RT-PCR) was used to detect the brain CREB gene expression .Results Compared with that of control group , the CREB gene expression was decreased in brain aging mice and brain aging +high fat diet group (P<0.05).There was not significant difference of CREB gene expression between brain ag-ing+exercise group and control group .The CREB gene expression in brain aging +high fat diet +exercise group was decreased significantly compared with that of normal group , but compared with that of brain aging mice , there was not significantly difference .Compared with that of control group , there was not remarkable effect in exercise group , but the CREB gene expression in high fat diet group was lower than that in normal control group(P<0.05).Conclusions High fat diet could aggravate brain aging , but exercise could prevent this effect by increasing CREB gene expression in brain aging mice .