CAJ | 학술논문

本文旨在观察噬菌体对黏质沙雷菌感染小鼠的治疗作用，为噬菌体疗法应用于细菌性感染提供依据。以黏质沙雷菌为宿主菌，采用双层琼脂噬斑法从污水中分离和纯化裂解性噬菌体。将最小致死量的黏质沙雷菌经腹腔感染BALB／c小鼠后，立即腹腔注射不同剂量的噬菌体，观察动物的生存率并确定噬菌体的保护剂量。在动物感染后的不同时间（0、20、40、60和180 min ）观察噬菌体疗法对动物存活率的影响。将噬菌体和细菌同时或分别注射动物后，分析噬菌体在动物体内的药代动力学。结果显示，经噬斑法从污水中分离出1株裂解性噬菌体（命名为φSM9‐3Y ），电镜观察发现该噬菌体属有尾噬菌体目肌尾噬菌体科。动物腹腔感染黏质沙雷菌并立即给予噬菌体后发现，当噬菌体的保护剂量为108 PFU／ml时，动物的存活率为100％。动物感染后40和60 min给予噬菌体（1010 PFU／ml）治疗，动物的存活率为60％。药代动力学表明，将噬菌体和细菌同时注入动物体内，在6 h内噬菌体的滴度维持在1010 PFU／ml。结果提示，噬菌体对黏质沙雷菌所致动物腹腔内感染的治疗是有效的，提示针对细菌性感染的噬菌体疗法具有潜在的应用价值。
본문지재관찰서균체대점질사뢰균감염소서적치료작용，위서균체요법응용우세균성감염제공의거。이점질사뢰균위숙주균，채용쌍층경지서반법종오수중분리화순화렬해성서균체。장최소치사량적점질사뢰균경복강감염BALB／c소서후，립즉복강주사불동제량적서균체，관찰동물적생존솔병학정서균체적보호제량。재동물감염후적불동시간（0、20、40、60화180 min ）관찰서균체요법대동물존활솔적영향。장서균체화세균동시혹분별주사동물후，분석서균체재동물체내적약대동역학。결과현시，경서반법종오수중분리출1주렬해성서균체（명명위φSM9‐3Y ），전경관찰발현해서균체속유미서균체목기미서균체과。동물복강감염점질사뢰균병립즉급여서균체후발현，당서균체적보호제량위108 PFU／ml시，동물적존활솔위100％。동물감염후40화60 min급여서균체（1010 PFU／ml）치료，동물적존활솔위60％。약대동역학표명，장서균체화세균동시주입동물체내，재6 h내서균체적적도유지재1010 PFU／ml。결과제시，서균체대점질사뢰균소치동물복강내감염적치료시유효적，제시침대세균성감염적서균체요법구유잠재적응용개치。
This study aims to evaluate the efficacy of phage therapy against Serratia marcescens infections in mice and to provide the basis of phage therapy applied in bacterial infections .Double‐agar overlay plaque method was employed to screen lytic phages from sewage , using Serratia marcescens isolates as hosts . Serratia marcescens strains at minimal lethal dose (MLD) were injected intraperitoneally (i .p .) into BALB/c mice and an i .p .of phage was followed .The survival rate of animals and protective dose of phage were examined at different time points (0 , 20 , 40 , 60 and 180 min ) after the bacterial challenge . Pharmacokinetics of phages injected alone or with bacteria was analyzed respectively .The results showed that a lytic phage ,designated φSM9‐3Y ,was isolated and characterized from sewage .Electron microscope revealed that phage φSM9‐3Y was in Siphoviridae family ,Caudovirales order .After injection of Serratia marcescens isolates ,immediate phage therapy at dose of 108 PFU/ml reached a protection rate of 100% . The protection rate was around 60% with a phage therapy (1010 PFU/ml ) delivered 60 min after the infection .Pharmacokinetics analysis showed that phage titer in blood was maintained at level of 1010 PFU/ml within 6 h when phages were injected i .p .together with bacteria .These data indicate that phages can save animals from pathogenic Serratia marcescens infection and suggest that phage therapy may be potentially used for the control of bacterial infections .