CAJ | 학술논문

采用乙醇回流法提取丹参、赤芍等药材，加入辅料（蔗糖∶糊精＝2∶1）制备冠心颗粒，采用RP-HPLC法测定颗粒的有效成分芍药苷、丹参素、丹酚酸B和丹酚酸A，以Agilent TC-C18（150 mm ×4．6 mm，5μm）为分析柱，以甲醇-0．2％磷酸溶液为流动相梯度洗脱，流速1．0 mL／min，柱温28℃。测得芍药苷、丹参素、丹酚酸B、丹酚酸A分别在5．44～87．04，6．68～106．88，9．48～151．68，3．06～48．96μg／mL内线性良好，r≥0．9992。
채용을순회류법제취단삼、적작등약재，가입보료（자당∶호정＝2∶1）제비관심과립，채용RP-HPLC법측정과립적유효성분작약감、단삼소、단분산B화단분산A，이Agilent TC-C18（150 mm ×4．6 mm，5μm）위분석주，이갑순-0．2％린산용액위류동상제도세탈，류속1．0 mL／min，주온28℃。측득작약감、단삼소、단분산B、단분산A분별재5．44～87．04，6．68～106．88，9．48～151．68，3．06～48．96μg／mL내선성량호，r≥0．9992。
Guanxin particles were prepared by adding different proportions sucrose and dextrin (2∶1) into the extractives from ethanol backflow extraction .The effective components such as paeoniflorin ,Danshen-su,salvianolic acid B and A were determined using RP-HPLC.The Agilent TC-C18 (150 mm ×4.6 mm, 5 μm) column was used,with methanol-0.2%phosphoric acid solution as mobile phase and gradient elu-tion.The flow rate was 1.0 mL/min at 28 ℃.The linear relationships were good in the range of 5.44~87.04 μg/mL for paeoniflorin,6.68~106.88 μg/mL for Danshensu,9.48~151.68 μg/mL for salvian-olic acid B and 3.06~48.96μg/mL for salvianolic acid A,respectively.The correlation coefficients were no less than 0.999 2.