CAJ | 학술논문

目的：探讨高脂环境下大鼠骨髓间充质干细胞（ BMSCs ）成骨分化过程中Wnt 信号通路相关基因的表达。方法采用第三代Wister大鼠BMSCs向成骨诱导。实验分两组：高脂组、对照组。培养期间观察两组细胞形态变化。成骨诱导培养7d，14d，21d采用RT－PCR 检测两组细胞Wnt 信号传导通路相关因子低密度脂蛋白受体相关蛋白5（LRP－5）、β连环蛋白（β－catenin）的表达，以及脂肪代谢因子成脂基因过氧化物酶体增殖子活化受体－γ（ PPAR－γ）的表达。结果 RT－PCR检测显示，在7d，14d，21d高脂组均较对照组LRP－5，β－catenin表达低（P＜0．05），PPAR－γ表达高（P＜0．05）。结论高脂环境下可诱发大鼠BMSCs向脂肪细胞分化，成骨细胞分化减少，Wnt 信号通路受到抑制。
목적：탐토고지배경하대서골수간충질간세포（ BMSCs ）성골분화과정중Wnt 신호통로상관기인적표체。방법채용제삼대Wister대서BMSCs향성골유도。실험분량조：고지조、대조조。배양기간관찰량조세포형태변화。성골유도배양7d，14d，21d채용RT－PCR 검측량조세포Wnt 신호전도통로상관인자저밀도지단백수체상관단백5（LRP－5）、β련배단백（β－catenin）적표체，이급지방대사인자성지기인과양화물매체증식자활화수체－γ（ PPAR－γ）적표체。결과 RT－PCR검측현시，재7d，14d，21d고지조균교대조조LRP－5，β－catenin표체저（P＜0．05），PPAR－γ표체고（P＜0．05）。결론고지배경하가유발대서BMSCs향지방세포분화，성골세포분화감소，Wnt 신호통로수도억제。
Objective To investigate the expression changes in the Wnt signaling pathway related factors in hyperlipidemia environment during the differentiation of rat bone marrow mesenchymal stem cells ( BMSCs ) into osteo-blasts .Methods The third generation BMSCs were used and induced to osteoblast .The experiment was divided into two groups:high fat group and control group .RT-PCR was used to evaluate the expression level of mRNA of LRP-5 ,β-cate-nin and PPAR-γat the 7days,14days,21 days.Results RT-PCR detection showed that at the 7days,14days,21 days LRP-5,β-catenin expression decreased in high fat group (P<0.05),and PPAR-γrised(P<0.05).Conclusion In the high lipid environment , BMSCs can induce adipocyte differentiation and reduce osteoblast differentiation , Wnt signal pathway is inhibited .