CAJ | 학술논문

目的观察美洲大蠊提取液（ ACE ）对大鼠溃疡性结肠炎（ UC ）的治疗效果及对大鼠结肠黏膜表皮生长因子（EGF）、胰岛素样生长因子－1（IGF－1）表达的影响，并与地塞米松作比较。方法用2，4，6－三硝基苯磺酸（TNBS）灌肠制作大鼠UC模型，取40只Sprague Dawley大鼠，随机均分为ACE组、地塞米松组、模型对照组、正常对照组4组，每组10只。造模并干预性治疗10d后观察每组大鼠疾病活动指数（DAI）及肠黏膜损伤指数评分（CMDI），并检测结肠黏膜组织中EGF及IGF－1的表达水平。结果<br>　　与模型对照组相比，ACE及地塞米松组DAI评分及CMDI评分显著降低（P＜0．01）；与地塞米松组相比，ACE组DAI评分较高（P＜0．05），而CMDI评分差距无统计学意义（P＞0．05）。与模型对照组对比，ACE组及地塞米松组的EGF表达水平升高（P＜0．05）， ACE组与地塞米松组IGF－1表达水平均降低（P＜0．05）。结论 ACE对TNBS诱导的大鼠UC有治疗作用，其作用较地塞米松弱，上调EGF及下调IGF－1的表达，可能为其治疗机制之一。
목적관찰미주대렴제취액（ ACE ）대대서궤양성결장염（ UC ）적치료효과급대대서결장점막표피생장인자（EGF）、이도소양생장인자－1（IGF－1）표체적영향，병여지새미송작비교。방법용2，4，6－삼초기분광산（TNBS）관장제작대서UC모형，취40지Sprague Dawley대서，수궤균분위ACE조、지새미송조、모형대조조、정상대조조4조，매조10지。조모병간예성치료10d후관찰매조대서질병활동지수（DAI）급장점막손상지수평분（CMDI），병검측결장점막조직중EGF급IGF－1적표체수평。결과<br>　　여모형대조조상비，ACE급지새미송조DAI평분급CMDI평분현저강저（P＜0．01）；여지새미송조상비，ACE조DAI평분교고（P＜0．05），이CMDI평분차거무통계학의의（P＞0．05）。여모형대조조대비，ACE조급지새미송조적EGF표체수평승고（P＜0．05）， ACE조여지새미송조IGF－1표체수평균강저（P＜0．05）。결론 ACE대TNBS유도적대서UC유치료작용，기작용교지새미송약，상조EGF급하조IGF－1적표체，가능위기치료궤제지일。
Objective To observe the therapeutic effects and the expression of Epidermal Growth Factor ( EGF) and Insulin-like Growth Factors-1(IGF-1) of America Cockroach Extract(ACE)on ulcerative colitis of rats,and to compare the effects of ACE on UC with Dexamethasone .Methods Forty healthy Sprague-Dawley rats were randomly divided into normal control group , model control group , ACE group and Dexamethasone group ,with 10 rats in each group .Colitis was induced by rectal administration of TNBS in the model control group , ACE group and Dexamethasone group .Rats in the ACE group were treated with ACE ,and the Dexamethasone group were treated with Dexam-ethasone.Disease Activity Index(DAI) and Colon Macroscopic Damage Index (CMDI) were calculated,and the expressions of EGF and IGF-1 in colon tissues were detected .Results Compared with the model control ,the DAI and CMDI scores in ACE and Dexamethasone group were decreased significantly (P<0.01).The EGF expression level in ACE group were increased (P<0.05),the IGF-1 expression level in ACE group and Dexamethasone group were decreased (P<0.05).Conclusion ACE has therapeutic effect for the UC of rats ,but its effect is relatively weak when compared with dexamethasone .The increased expression of EGF and decreased expression of IGF-1 maybe one of the possible mechanisms .