CAJ | 학술논문

目的：检测未转染的毛乳头细胞第3、6、9代雄激素受体（androgen receptor，AR）蛋白的表达；构建及鉴定AR基因过表达的腺病毒载体，将其转染进入人毛乳头细胞（dermal papilla cell，DPC），探讨AR基因对人DPC增生的影响。方法应用基因同源重组获得pAdeasy-Adtrack-AR重组腺病毒质粒，腺病毒感染人DPC，采用蛋白质印迹法（Western blot）检测AR的表达，应用CCK-8法检测人DPC的增生。结果未转染前，DPC的AR表达量随着传代逐渐降低；成功构建了AR基因腺病毒载体，腺病毒转染人DPC 48 h后AR蛋白高表达，腺病毒转染24、48、72 h对人DPC的增生无影响。结论人DPC过表达AR基因模型为进一步研究雄激素性脱发提供了一定的实验基础。
목적：검측미전염적모유두세포제3、6、9대웅격소수체（androgen receptor，AR）단백적표체；구건급감정AR기인과표체적선병독재체，장기전염진입인모유두세포（dermal papilla cell，DPC），탐토AR기인대인DPC증생적영향。방법응용기인동원중조획득pAdeasy-Adtrack-AR중조선병독질립，선병독감염인DPC，채용단백질인적법（Western blot）검측AR적표체，응용CCK-8법검측인DPC적증생。결과미전염전，DPC적AR표체량수착전대축점강저；성공구건료AR기인선병독재체，선병독전염인DPC 48 h후AR단백고표체，선병독전염24、48、72 h대인DPC적증생무영향。결론인DPC과표체AR기인모형위진일보연구웅격소성탈발제공료일정적실험기출。
ObjectiveTo detect the expression of androgen receptor (AR) before transfection; and to construct the adenoviral overexpressing vectors of AR and investigate its effects on proliferation of human dermal papilla cells (DPCs) after transfection. MethodsThe AR adenoviral vectors were constructed by using gene recombination technique, then the expression of AR was detected by western bolt, and the vectors’ effects on proliferation of DPCs was detected by CCK-8 assay.ResultsBefore transfection, AR expression was gradually reduced with the passage of DPCs. The AR adenoviral vectors were successfully constructed. The AR expression in DPCs was increased after transfection. The AR adenoviral vectors did not effect the proliferation of DPCs.ConclusionIt’s important to construct an AR overexpressing model, which provides some experimental basis for further study of alopecia androgenetic.