实验与检验医学
實驗與檢驗醫學
실험여검험의학
EXPERIMENTAL AND LABORATORY MEDICINE
2015年
2期
147-149,160
,共4页
急性髓系白血病%miR-335%诊断
急性髓繫白血病%miR-335%診斷
급성수계백혈병%miR-335%진단
Acute myeloid leukemia%miR-335%Diagnosis%Acute leukemia
目的：探讨急性髓系白血病(AML)患者循环miR-335的表达及其临床意义。方法本研究共纳入26例初诊AML患者和26例健康对照者，取外周血通过实时荧光定量PCR检测miR-335表达量并分析其与AML临床资料的相关性。绘制ROC曲线、计算曲线下面积以评估miR-335对新发AML的诊断价值。结果循环miRNA-335相对表达量在同组内纳入研究对象的年龄、性别比较上未见明显差异(P>0.05)。与AML患者外周血白细胞计数及原始细胞计数无显著相关性(P>0.05)。与骨髓原始细胞计数及FAB分型无显著相关性(P>0.05)。当以相对表达量2.7为cutoff值时， miRNA-335诊断AML的AUC可达0.915(95%可信区间：0.828~1.000；P<0.0001)，miRNA-335诊断AML的敏感性可达80.8%，特异性可达100%。结论血清循环miR-335表达量在AML患者中显著升高，可作为AML辅助诊断标志物。
目的：探討急性髓繫白血病(AML)患者循環miR-335的錶達及其臨床意義。方法本研究共納入26例初診AML患者和26例健康對照者，取外週血通過實時熒光定量PCR檢測miR-335錶達量併分析其與AML臨床資料的相關性。繪製ROC麯線、計算麯線下麵積以評估miR-335對新髮AML的診斷價值。結果循環miRNA-335相對錶達量在同組內納入研究對象的年齡、性彆比較上未見明顯差異(P>0.05)。與AML患者外週血白細胞計數及原始細胞計數無顯著相關性(P>0.05)。與骨髓原始細胞計數及FAB分型無顯著相關性(P>0.05)。噹以相對錶達量2.7為cutoff值時， miRNA-335診斷AML的AUC可達0.915(95%可信區間：0.828~1.000；P<0.0001)，miRNA-335診斷AML的敏感性可達80.8%，特異性可達100%。結論血清循環miR-335錶達量在AML患者中顯著升高，可作為AML輔助診斷標誌物。
목적：탐토급성수계백혈병(AML)환자순배miR-335적표체급기림상의의。방법본연구공납입26례초진AML환자화26례건강대조자，취외주혈통과실시형광정량PCR검측miR-335표체량병분석기여AML림상자료적상관성。회제ROC곡선、계산곡선하면적이평고miR-335대신발AML적진단개치。결과순배miRNA-335상대표체량재동조내납입연구대상적년령、성별비교상미견명현차이(P>0.05)。여AML환자외주혈백세포계수급원시세포계수무현저상관성(P>0.05)。여골수원시세포계수급FAB분형무현저상관성(P>0.05)。당이상대표체량2.7위cutoff치시， miRNA-335진단AML적AUC가체0.915(95%가신구간：0.828~1.000；P<0.0001)，miRNA-335진단AML적민감성가체80.8%，특이성가체100%。결론혈청순배miR-335표체량재AML환자중현저승고，가작위AML보조진단표지물。
Objective To investigate the expression and clinical significance of circulating miR-335 in acute myeloid leukemi-a (AML) patients. Methods Twenty-six newly diagnosed AML patients and 26 healthy controls were enrolled in our study.The ex-pression of miR-335 in periphral blood of AML patients was detected by Real-time quantitative PCR and its correlation with clinical material of AML was analyzed. Then the receiver operating characteristic curve (ROC) was conducted, and the area under the curve (AUC) was used to evaluate the diagnostic accuracy of miR-335 as a potential biomarker in the new AML. Results There was no significant difference of the relative expression level of circulating miR-335 with the subjects' age and sex within the same group (P>0.05). There was no significant difference of the white blood cell count and primitive cell count in periphral blood of AML (P>0.05), the same as the primitive bone marrow cell count and FAB classification in AML (P>0.05). ROC curve analyses revealed an AUC of 0.915 in diagnosing AML. When relative expression 2.7 used as the cut off value of miR-335, the sensitivity and specificity of miR-335 in diagnosing AML could reached 80.8% and 100%. Conclusion The expression of circulating miR-335 significantly increased in AML patients, which could be an auxiliary diagnostic biomarker for AML.
