CAJ | 학술논문

目的：研究柴胡水提组分对大鼠发热模型的解热作用及伴随的毒副作用，为其“功效-毒性”相关性研究提供实验依据。方法采用背部皮下注射2，4-二硝基苯酚，制备大鼠发热模型，灌胃不同剂量柴胡水提组分，每30 min 测体温1次，连续测定4 h，记录肛温，观察其解热作用；检测血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆汁酸（TBA）、总胆红素（TBIL)、肌酐（Cr）、尿素氮（BUN）的活性及含量，剖取心脏、肝脏、肾脏称重，计算心、肝、肾各脏体比值，评价其药效伴随毒副作用。结果柴胡水提组分对2,4-二硝基苯酚所致大鼠发热模型有降温作用，给药3 d 后血清 ALT、AST 活性明显升高，肝体比值无明显变化。结论药效剂量的柴胡水提组分对大鼠发热模型有一定的降温作用，同时会产生一定的肝毒性，并呈现一定的剂量依赖关系。
목적：연구시호수제조분대대서발열모형적해열작용급반수적독부작용，위기“공효-독성”상관성연구제공실험의거。방법채용배부피하주사2，4-이초기분분，제비대서발열모형，관위불동제량시호수제조분，매30 min 측체온1차，련속측정4 h，기록항온，관찰기해열작용；검측혈청중병안산안기전이매（ALT）、천문동안산안기전이매（AST）、총담즙산（TBA）、총담홍소（TBIL)、기항（Cr）、뇨소담（BUN）적활성급함량，부취심장、간장、신장칭중，계산심、간、신각장체비치，평개기약효반수독부작용。결과시호수제조분대2,4-이초기분분소치대서발열모형유강온작용，급약3 d 후혈청 ALT、AST 활성명현승고，간체비치무명현변화。결론약효제량적시호수제조분대대서발열모형유일정적강온작용，동시회산생일정적간독성，병정현일정적제량의뢰관계。
Objective To study the antipyretic effects and accompanied side effects of water extraction of Bupleurum components for rat fever model so as to provide the experimental basis for "efficacy- toxicity" correlation. Methods Rat fever model was established via the back injection of 2,4-dinitrophenol (DNP). Different doses of water extraction of Bupleurum components were given to rats by gavage. The temperature was detected in anus once every 30 minutes, continuously determined for 4 hours, recorded anal temperature and observed the antipyretic effect. At the same time, the levels of ALT, AST, TBA, TBIL, Cr and BUN in serum were detected and the heart, liver and kidney indexes were calculated. Efficacy and accompanied side effects were evaluated via all these data. Results Antipyretic effect was significantly reflected when rat fever model caused by DNP were treated with water extraction of Bupleurum components by gavage. The activities of ALT and AST increased obviously after administration for consecutive 3 days. Conclusion Water extraction of Bupleurum components has the effect of dropping in temperature to the rat fever model, which is accompanied by hepatotoxicity. The effect and hepatotoxicity all show a certain dose dependence relationships.