中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2015年
5期
48-50
,共3页
曲安奈德新霉素贴膏%醋酸曲安奈德%HPLC%含量测定
麯安奈德新黴素貼膏%醋痠麯安奈德%HPLC%含量測定
곡안내덕신매소첩고%작산곡안내덕%HPLC%함량측정
Triamcinolone acetonide and neomycin paste%Triamcinolone acetonide acetate%HPLC%Determination
目的：建立曲安奈德新霉素贴膏中醋酸曲安奈德含量的HPLC测定方法。方法采用反相高效液相色谱法，使用DIONEX Acclaim 120 C18（250mm×4.6mm，5μm）色谱柱，以甲醇-水（60∶40）作为流动相，检测波长240nm，流速1.0mL/min，柱温为30℃。结果醋酸曲安奈德在0.05～0.50μg范围内线性较好（r=0.9999）,平均回收率为98.09%（RSD为0.73%）。结论该方法快速、简便、可靠、准确度高、重复性好，处方中其他成分对测定亦无干扰，分离效果好，可用于曲安奈德新霉素贴膏中醋酸曲安奈德含量测定。
目的：建立麯安奈德新黴素貼膏中醋痠麯安奈德含量的HPLC測定方法。方法採用反相高效液相色譜法，使用DIONEX Acclaim 120 C18（250mm×4.6mm，5μm）色譜柱，以甲醇-水（60∶40）作為流動相，檢測波長240nm，流速1.0mL/min，柱溫為30℃。結果醋痠麯安奈德在0.05～0.50μg範圍內線性較好（r=0.9999）,平均迴收率為98.09%（RSD為0.73%）。結論該方法快速、簡便、可靠、準確度高、重複性好，處方中其他成分對測定亦無榦擾，分離效果好，可用于麯安奈德新黴素貼膏中醋痠麯安奈德含量測定。
목적：건립곡안내덕신매소첩고중작산곡안내덕함량적HPLC측정방법。방법채용반상고효액상색보법，사용DIONEX Acclaim 120 C18（250mm×4.6mm，5μm）색보주，이갑순-수（60∶40）작위류동상，검측파장240nm，류속1.0mL/min，주온위30℃。결과작산곡안내덕재0.05～0.50μg범위내선성교호（r=0.9999）,평균회수솔위98.09%（RSD위0.73%）。결론해방법쾌속、간편、가고、준학도고、중복성호，처방중기타성분대측정역무간우，분리효과호，가용우곡안내덕신매소첩고중작산곡안내덕함량측정。
ObjectiveTo establish a method for content determination of triamcinolone acetonide acetate in triamcinolone acetonide and neomycin paste by HPLC.Methods The experimental condition of the RP-HPLC method was as follows:DIONEX Acclaim 120 C18(250mm×4.6mm,5μm),with linear gradient elution using methanol and water (60∶40),The detected wavelength was 240 nm,the flow rate was 1.0 ml/min,The column temperature was at 30℃. ResultsThe fluocinolone acetonide curve was linear in the range of 0.05~0.50μg(r=0.9999).The average recovery rate was 98.09% withRSD of 0.73%.ConclusionThe method is simple,reliable,accurate and It could be used in the determination of triamcinolone acetonide acetate in triamcinolone acetonide and neomyc in paste.
