CAJ | 학술논문

目的：探讨超声引导介入治疗卵巢子宫内膜样囊肿前、后患者血清中的差异表达因子。方法选取2011年12月至2013年12月在哈尔滨医科大学附属第四医院治疗的9例卵巢子宫内膜样囊肿患者，全部患者均在超声引导下介入治疗，治疗前及治疗后3个月分别采集患者血清标本，行NimbleGen全基因组表达谱芯片检测，分析治疗前、后血清中卵巢子宫内膜样囊肿相关因子的基因功能及信号通路，筛选出表达显著差异的因子。结果通过NimbleGen全基因组表达谱芯片检测筛选出CD36、NR4A1、HES1因子在卵巢子宫内膜样囊肿介入治疗后表达下调。CD36、NR4A1、HES1参与的血管化作用、趋化作用、细胞粘连作用等基因功能与卵巢子宫内膜样囊肿的发病机制相关， CD36、NR4A1、HES1通过脂肪细胞因子通路、促分裂原活化蛋白激酶信号通路、青春晚期糖尿病通路发挥作用。结论卵巢子宫内膜样囊肿患者血清CD36、NR4A1、HES1因子在超声引导下乙醇硬化介入治疗后表达下调，参与卵巢子宫内膜样囊肿的发病机制。
목적：탐토초성인도개입치료란소자궁내막양낭종전、후환자혈청중적차이표체인자。방법선취2011년12월지2013년12월재합이빈의과대학부속제사의원치료적9례란소자궁내막양낭종환자，전부환자균재초성인도하개입치료，치료전급치료후3개월분별채집환자혈청표본，행NimbleGen전기인조표체보심편검측，분석치료전、후혈청중란소자궁내막양낭종상관인자적기인공능급신호통로，사선출표체현저차이적인자。결과통과NimbleGen전기인조표체보심편검측사선출CD36、NR4A1、HES1인자재란소자궁내막양낭종개입치료후표체하조。CD36、NR4A1、HES1삼여적혈관화작용、추화작용、세포점련작용등기인공능여란소자궁내막양낭종적발병궤제상관， CD36、NR4A1、HES1통과지방세포인자통로、촉분렬원활화단백격매신호통로、청춘만기당뇨병통로발휘작용。결론란소자궁내막양낭종환자혈청CD36、NR4A1、HES1인자재초성인도하을순경화개입치료후표체하조，삼여란소자궁내막양낭종적발병궤제。
ObjectiveTo explore changes of immune factors in serum before and after ultrasound-guided (US-guided ) interventional treatment of ovarian endometrial cyst.MethodsNine patients with ovarian endometrial cyst were selected between December 2011 and December 2013 in the fourth affiliated hospital of Harbin Medical University. All patients performed US-guided interventional treatment, the serum specimens were collected before and after three months of treatment respectively. NimbleGen genome microarray of serum specimens were performed to analysis the gene function and gene pathways of related factors of ovarian endometrial cyst and to find out factors with significant expression differences before and after treatment. The relevance between factors with significant expression differences and the pathogenesis of ovarian endometrial cyst were analyzed.ResultsThe expression of CD36, NR4A1and HES1 were found to be down-regulated after US-guided interventional treatment of ovarian endometrial cyst by NimbleGen genome microarray. The gene function of CD36, NR4A1 and HES1participating in angiogenesis, chemotaxis, cell adhesion was relevant to the pathogenesis of the ovarian endometrial cyst, and played roles through Adipocytokine signaling pathway, MAPK signalig pathway, Maturity onset diabetes of the young signaling pathways.ConclusionSerum CD36, NR4A1 and HES1of ovarian endometrial cyst were down-regulated after US-guided ethanol sclerotherapy , and involved in the pathogenesis of ovarian endometrial cyst .