CAJ | 학술논문

目的：建立HPLC法同时测定三七花盘中人参皂苷Rb1和人参皂苷Rb3的含量。方法采用ACE 5 C18（250mm×4.6mm，5μm）色谱柱；流动相：乙腈-0.05%磷酸溶液（32∶68）；流速：1.0mL/min；检测波长：203nm；柱温：25℃。结果人参皂苷Rb1和人参皂苷Rb3分别在10.25～143.50mg/L（r=0.9993， n=7）和10.94～153.16mg/L（r=0.9997，n=7）范围内呈良好线性关系；平均回收率（n=6）分别为98.78%和98.17%。结论本测定方法简便、快速、准确，为三七花盘的综合开发利用提供了参考，有利于控制三七花盘的质量。
목적：건립HPLC법동시측정삼칠화반중인삼조감Rb1화인삼조감Rb3적함량。방법채용ACE 5 C18（250mm×4.6mm，5μm）색보주；류동상：을정-0.05%린산용액（32∶68）；류속：1.0mL/min；검측파장：203nm；주온：25℃。결과인삼조감Rb1화인삼조감Rb3분별재10.25～143.50mg/L（r=0.9993， n=7）화10.94～153.16mg/L（r=0.9997，n=7）범위내정량호선성관계；평균회수솔（n=6）분별위98.78%화98.17%。결론본측정방법간편、쾌속、준학，위삼칠화반적종합개발이용제공료삼고，유리우공제삼칠화반적질량。
Objective To establish a high performance liquid chromatographic(HPLC)method for simultaneous detection of ginsenoside Rb1 and ginsenoside Rb3 in flower disc of Panax notoginseng.Methods ACE5 C18 column (250mm×4.6mm,5μm)was used with a mixture of acetonitrile-0.05% phosphoric acid(32∶68,V:V)as the mobile phase.The flow rate was 1.0mL/min,the detection wavelength of 203nm,the column temperature of 25℃.Results The linear range of ginsenoside Rb1 and ginsenoside Rb3 were 10.25~143.50mg/L(r=0.9993,n=7)and 10.94~153.16 mg/L (r=0.9997,n=7)respectively.The average recovery rates were 98.78% and 98.17%,respectively.ConclusionThe method is simple,accurate and sensitive with good reproducibility.It can provide references for resources development and be used for quality controlling of flower disc of Panax notoginseng.