CAJ | 학술논문

目的：建立同时测定拉莫三嗪、苯巴比妥、苯妥英、卡马西平和氯硝西泮血清浓度的HPLC法。方法血清样品经甲醇沉淀蛋白后直接进样分析，色谱柱为Waters C18（5μm，4.6×250mm）柱，流动相为甲醇-水（55∶45），检测波长235nm。结果在一定浓度范围内(拉莫三嗪：1.3～50.0μg/mL；苯巴比妥：2.5～100.0μg/mL；苯妥英：2.2～70.0μg/mL；卡马西平：1.8～35.0μg/mL；氯硝西泮：2.5～80.0μg/mL），各药物的峰面积与浓度呈良好的线性关系。低、中、高浓度的质控样品回收率均高于95%，相对标准差（RSD）均小于10%。结论本方法操作简便，结果稳定可靠，适用于临床进行血药浓度监测。
목적：건립동시측정랍막삼진、분파비타、분타영、잡마서평화록초서반혈청농도적HPLC법。방법혈청양품경갑순침정단백후직접진양분석，색보주위Waters C18（5μm，4.6×250mm）주，류동상위갑순-수（55∶45），검측파장235nm。결과재일정농도범위내(랍막삼진：1.3～50.0μg/mL；분파비타：2.5～100.0μg/mL；분타영：2.2～70.0μg/mL；잡마서평：1.8～35.0μg/mL；록초서반：2.5～80.0μg/mL），각약물적봉면적여농도정량호적선성관계。저、중、고농도적질공양품회수솔균고우95%，상대표준차（RSD）균소우10%。결론본방법조작간편，결과은정가고，괄용우림상진행혈약농도감측。
Objective To develop a new method for simultaneously determining Lamotrigine, phenobarbital, phenytoin, carbamazepine and clonazepam in human serum by HPLC.MethodsSerum samples were analyzed after protein deposition by methanol.The HPLC method was performed on the column of Waters C18 (5μm, 4.6×250mm) with mobile phase consisted of ethanol-water (55∶45), the UV detection wavelength was 235nm.Results In a certain range of concentration (lamotrigine: 1.3-50.0μg/mL; phenobarbital: 2.5-100.0μg/mL; phenyltoin: 2.2-70.0μg/mL; carbamazepine: 1.8-35.0μg/mL; clonazepam: 2.5-80.0μg/mL), there was a good linear relationship between peak area and concentration of each drug. Low、medium and high concentration of the quality control sample recovery rate was higher than 95%, the relative standard deviation (RSD) is less than 10%.ConclusionThe method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of 5 compounds which is suitable for therapeutic drug monitoring.