癫癎与神经电生理学杂志
癲癎與神經電生理學雜誌
전간여신경전생이학잡지
JOURNAL OF EPILEPTOLOGY AND ELECTRONEUROPHYSIOLOGY(CHINA)
2015年
2期
65-68
,共4页
杏仁核%电刺激%点燃%癫?模型
杏仁覈%電刺激%點燃%癲?模型
행인핵%전자격%점연%전?모형
amygdala%electrical stimulation%kindling%epilepsy model
目的：建立一种快速稳定的杏仁核电刺激点燃癫?模型。方法：选取SD雄性大鼠40只制作杏仁核点燃癫?模型，按随机化原则分为5组：A 组为空白对照组；B组刺激频率16 Hz ，波宽1．0ms、强度0．5mA，串长10个；C组刺激频率16Hz、波宽1．0ms，强度0．5mA，串长160个；D组刺激频率60Hz，波宽1．0ms，强度0．5mA，串长10个；E组采用刺激频率60Hz，波宽1．0ms，强度0．5mA，串长160个进行实验。利用这种不同频率、强度、串长电刺激杏仁核的模式寻找出最快速有效且稳定的点燃癫?模型的方法。结果：在全部大鼠中成功点燃23只（点燃成功率为72％），然而在4种不同电刺激方法中，B组、C组、D组、E组的点燃成功率分别为100％、87％、87％和11％，B组与各组及组间比较差异有统计学意义（P＜0．01）。结论：本实验大鼠杏仁核电刺激可快速建立癫?模型，采用刺激频率16Hz、波宽1．0ms、强度0．5mA、串长10个的刺激参数，杏仁核快速点燃的效果最好。
目的：建立一種快速穩定的杏仁覈電刺激點燃癲?模型。方法：選取SD雄性大鼠40隻製作杏仁覈點燃癲?模型，按隨機化原則分為5組：A 組為空白對照組；B組刺激頻率16 Hz ，波寬1．0ms、彊度0．5mA，串長10箇；C組刺激頻率16Hz、波寬1．0ms，彊度0．5mA，串長160箇；D組刺激頻率60Hz，波寬1．0ms，彊度0．5mA，串長10箇；E組採用刺激頻率60Hz，波寬1．0ms，彊度0．5mA，串長160箇進行實驗。利用這種不同頻率、彊度、串長電刺激杏仁覈的模式尋找齣最快速有效且穩定的點燃癲?模型的方法。結果：在全部大鼠中成功點燃23隻（點燃成功率為72％），然而在4種不同電刺激方法中，B組、C組、D組、E組的點燃成功率分彆為100％、87％、87％和11％，B組與各組及組間比較差異有統計學意義（P＜0．01）。結論：本實驗大鼠杏仁覈電刺激可快速建立癲?模型，採用刺激頻率16Hz、波寬1．0ms、彊度0．5mA、串長10箇的刺激參數，杏仁覈快速點燃的效果最好。
목적：건립일충쾌속은정적행인핵전자격점연전?모형。방법：선취SD웅성대서40지제작행인핵점연전?모형，안수궤화원칙분위5조：A 조위공백대조조；B조자격빈솔16 Hz ，파관1．0ms、강도0．5mA，천장10개；C조자격빈솔16Hz、파관1．0ms，강도0．5mA，천장160개；D조자격빈솔60Hz，파관1．0ms，강도0．5mA，천장10개；E조채용자격빈솔60Hz，파관1．0ms，강도0．5mA，천장160개진행실험。이용저충불동빈솔、강도、천장전자격행인핵적모식심조출최쾌속유효차은정적점연전?모형적방법。결과：재전부대서중성공점연23지（점연성공솔위72％），연이재4충불동전자격방법중，B조、C조、D조、E조적점연성공솔분별위100％、87％、87％화11％，B조여각조급조간비교차이유통계학의의（P＜0．01）。결론：본실험대서행인핵전자격가쾌속건립전?모형，채용자격빈솔16Hz、파관1．0ms、강도0．5mA、천장10개적자격삼수，행인핵쾌속점연적효과최호。
Objective:To establish a rapid and stable amygdala kindling model of epilepsy .Methods:Forty SD male rats were selected to establish an amygdala kindling model of epilepsy .The rats were ran‐domly divided into 5 groups .Group A was the blank control group .Both Group B and Group C adopted the same stimulus frequencies of 16 Hz ,wave space of 1 .0 ms and intensity of 0 .5 mA .The only differ‐ence was the string length ,10 and 160 for Group B and Group C respectively .Similarly ,both Group D and Group E adopted the same stimulus frequency of 60 Hz ,wave length of 1 .0 ms and intensity of 0 .5 mA . Again the only difference was the string length ,l0 and 160 for Group D and Group E respectively .The aim was to find out the most rapid ,effective and stable method by using different stimulus frequency ,in‐tensity and string length to stimulate the amygdala .Results:Of all rats ,23 of them were successfully kin‐dled ,with a success rate of 72% .While among four different electric‐stimulated methods ,the success rate for Group B ,Group C ,Group D and Group E was 100% ,87% ,87% and 11% respectively .The differ‐ences between Group B and other groups and the differences within all the groups were of statistical sig‐nificance(P<0 .01) .Conclusion:The electric stimulation of amygdala in rats could can help establish a model of epilepsy .Adopting the parameters of Group B(stimulus frequency :16 Hz ,wave space of 1 .0 ms , intensity of 0 .5 mA ,duration:10 ms ,amygdala was rapidly kindled ,and the best results can be achieled .
