CAJ | 학술논문

目的：研究丝胶对2型糖尿病大鼠肝脏肝细胞核因子（ HNF）-4α表达的影响。方法雄性SD大鼠48只随机分为：正常对照组、糖尿病模型组、丝胶治疗组和丝胶预防组，每组12只大鼠。链脲佐菌素连续腹腔注射制作2型糖尿病大鼠模型，待模型成功建立后，模型组大鼠不做任何处理，丝胶治疗组大鼠给予2.4 g· kg－1· d－1的丝胶连续灌胃35 d；丝胶预防组大鼠在链脲佐菌素连续腹腔注射前，给予丝胶（2.4 g· kg－1· d－1）灌胃，时间与丝胶治疗组相同。分别采用Western 印迹法和RT-PCR法检测大鼠肝脏HNF-4α蛋白和mRNA的表达。结果与正常对照组大鼠比较，糖尿病模型组大鼠肝脏HNF-4α蛋白和mRNA的表达明显升高（ P＜0.01）；与糖尿病模型组大鼠比较，丝胶治疗组、丝胶预防组大鼠肝脏 HNF-4α蛋白和mRNA的表达明显降低（P＜0.01）。结论丝胶改善糖代谢的作用可能与其调节肝脏HNF-4α的表达有关。
목적：연구사효대2형당뇨병대서간장간세포핵인자（ HNF）-4α표체적영향。방법웅성SD대서48지수궤분위：정상대조조、당뇨병모형조、사효치료조화사효예방조，매조12지대서。련뇨좌균소련속복강주사제작2형당뇨병대서모형，대모형성공건립후，모형조대서불주임하처리，사효치료조대서급여2.4 g· kg－1· d－1적사효련속관위35 d；사효예방조대서재련뇨좌균소련속복강주사전，급여사효（2.4 g· kg－1· d－1）관위，시간여사효치료조상동。분별채용Western 인적법화RT-PCR법검측대서간장HNF-4α단백화mRNA적표체。결과여정상대조조대서비교，당뇨병모형조대서간장HNF-4α단백화mRNA적표체명현승고（ P＜0.01）；여당뇨병모형조대서비교，사효치료조、사효예방조대서간장 HNF-4α단백화mRNA적표체명현강저（P＜0.01）。결론사효개선당대사적작용가능여기조절간장HNF-4α적표체유관。
Objective To study the effects of sericin on hepatocyte nuclear factor 4 alpha ( HNF-4α) expression in liver of type 2 diabetesmellitusratsmodel.Methods 48maleSDratswererandomlydividedintothefollowing4groups:normalcontrol,diabetesmodel, sericin treatment and sericin prevention groups ( n=12 ).The type 2 diabetes model rats were induced by continuous intraperitoneal injection of streptozotocin.After successfully establishing the diabetes animal models ,the rats in diabetes model group were not given any more treat-ments.Rats in sericin treatment group were lavaged with sericin (2.4 g· kg-1 · d-1 ).Rats in sericin prevention group were lavaged with seri-cin (2.4 g· kg-1 · d-1 ) before injecting streptozotocin.Western blot and RT-PCR were used to detect the HNF-4αprotein and mRNA ex-pression in liver.Results The HNF-4αprotein and mRNA expression in liver of rats in model group were obviously higher than those of nor -mal control group ( P<0.01 ).The HNF-4αprotein and mRNA expression in liver of rats in sericin treatment group and sericin prevention group were obviously lower than those of model group ( P<0.01 ).Conclusions The effects of sericin on improving glucose metabolism may be associated with its down-regulating the HNF-4αexpression in liver.