CAJ | 학술논문

目的：建立2型糖尿病(type 2 diabetes mellitus,T2DM)致阿尔茨海默病(Alzheimer’s disease,AD)损伤大鼠模型。方法高脂高糖饮食持续喂养老年大鼠,建立 T2DM 大鼠模型,继而运用 Morris 水迷宫试验、脑脊液微透析及 ELISA 技术、电生理学检查方法筛选出继发 AD 损伤大鼠并病理鉴定。结果与单纯 T2DM 组及普通饮食组比较,T2DM+AD 组大鼠存在明显的学习、记忆功能障碍；T2DM+AD 组大鼠海马区域脑脊液中β淀粉样蛋白(β-AP)水平显著升高；且该组大鼠海马神经元灌流乙酰胆碱(Ach)所引发的动作电位频率显著降低。结论持续高脂高糖饮食喂养可成功建立2型糖尿病致阿尔茨海默病损伤大鼠模型。
목적：건립2형당뇨병(type 2 diabetes mellitus,T2DM)치아이자해묵병(Alzheimer’s disease,AD)손상대서모형。방법고지고당음식지속위양노년대서,건립 T2DM 대서모형,계이운용 Morris 수미궁시험、뇌척액미투석급 ELISA 기술、전생이학검사방법사선출계발 AD 손상대서병병리감정。결과여단순 T2DM 조급보통음식조비교,T2DM+AD 조대서존재명현적학습、기억공능장애；T2DM+AD 조대서해마구역뇌척액중β정분양단백(β-AP)수평현저승고；차해조대서해마신경원관류을선담감(Ach)소인발적동작전위빈솔현저강저。결론지속고지고당음식위양가성공건립2형당뇨병치아이자해묵병손상대서모형。
Objective To establish the rat model of type 2 diabetes mellitus (T2DM)-induced Alzheimer’s disease (AD).Methods The rat model of T2DM was established by continuous high fat and high glucose diet in aged rats. Then the rat model was screened and identified by using Morris water maze, cerebrospinal fluid microdialysis technique,ELISA,electrophysiological technique and pathologic method,respectively.Results Compared with the normal group and the T2DM group,the rats in T2DM+DM group had obviously learning and memory impairment;the level ofβ-AP in the hippocampus was significantly higher and the frequency of the spikes induced by Achin the hippocampus was notably lower.Conclusion The rat model of T2DM-induced AD can be successfully established by continuous high fat and high glucose diet in rats.