CAJ | 학술논문

目的：探讨 RNA 结合蛋白 La(La)对宫颈癌细胞侵袭转移的作用及其作用机制。方法采用 RNA 干扰技术沉默 La 基因在宫颈癌细胞株 HeLa 中的表达，并通过 G418筛选，构建稳定表达的 HeLa 的 La 干扰克隆。采用划痕实验、Transwell 小室等检测 La 对宫颈癌细胞侵袭转移的影响。利用 Western blot 的方法检测细胞中基质金属蛋白酶2(MMP-2)和基质金属蛋白酶抑制剂2(TIMP-2)的表达，并采用明胶酶谱的方法检测细胞培养上清中 MMP-2的活性。结果La 干扰后细胞的迁徙和侵袭能力明显降低，对细胞迁徙距离和穿过 Transwell 小室的细胞数目进行分析后发现，差异有统计学意义；同时发现在细胞中 MMP-2的蛋白表达水平及酶活性均降低，而 TIMP-2的蛋白表达水平上调。结论La 对宫颈癌的侵袭转移具有促进作用，这种作用可能与其对基质金属蛋白及其抑制剂的调控相关。
목적：탐토 RNA 결합단백 La(La)대궁경암세포침습전이적작용급기작용궤제。방법채용 RNA 간우기술침묵 La 기인재궁경암세포주 HeLa 중적표체，병통과 G418사선，구건은정표체적 HeLa 적 La 간우극륭。채용화흔실험、Transwell 소실등검측 La 대궁경암세포침습전이적영향。이용 Western blot 적방법검측세포중기질금속단백매2(MMP-2)화기질금속단백매억제제2(TIMP-2)적표체，병채용명효매보적방법검측세포배양상청중 MMP-2적활성。결과La 간우후세포적천사화침습능력명현강저，대세포천사거리화천과 Transwell 소실적세포수목진행분석후발현，차이유통계학의의；동시발현재세포중 MMP-2적단백표체수평급매활성균강저，이 TIMP-2적단백표체수평상조。결론La 대궁경암적침습전이구유촉진작용，저충작용가능여기대기질금속단백급기억제제적조공상관。
Objective To investigate the role of RNA-binding protein La protein in the invasion and migration of cervical cancer cells.Methods RNAi technology was used to silence the La protein in HeLa cell,a cell line of cervical cancer,and then screened by G418.Finally the stably expressed HeLa-shLa cell line was constructed and then wound healing,Transwell,Western blot and gelatin zymography assay were performed. Results After La protein HeLa was silenced by RNAi,the invasion and migration capabilities of HeLa cells were decreased significantly compared with those of the controls.Meanwhile,SiRNA-mediated depletion of La reduced the expression of MMP-2 and increased the expression of TIMP-2.Meanwhile the activity of MMP-2 was reduced too.Conclusion RNA-binding protein La promotes the invasion and migration of cervical cancer cells,which may be related to regulating its matrix metalloproteinases and inhibitors.