CAJ | 학술논문

目的：建立接近于人的猪同种异体左肺原位移植动物模型。方法环江香猪12只作为供体，巴马香猪12只作为受体，左侧第4肋间开胸，完成左肺原位移植。术后1、2、4、6、12 h开胸测左、右肺动脉的压力，同时取左、右肺静脉血进行血气分析，取左、右肺组织，观察含水量及病理学改变。结果动物术后均存活，随着术后时间延长，供肺静脉血氧合指数（ PaO2／FiO2）下降和肺动脉压（ PAP）上升，与受体正常肺比较，差异有显著性（ P＜0.05）。随着时间的推移，移植肺组织出现水肿、炎性细胞浸润、红细胞渗出，肺泡壁增厚明显，部分肺泡腔完全闭塞，部分肺组织实变等变化，与受体肺组织比较，含水量增加显著（ P＜0.05）。结论为研究肺移植缺血再灌注损伤及免疫排斥反应研究机制提供了理想的动物模型。
목적：건립접근우인적저동충이체좌폐원위이식동물모형。방법배강향저12지작위공체，파마향저12지작위수체，좌측제4륵간개흉，완성좌폐원위이식。술후1、2、4、6、12 h개흉측좌、우폐동맥적압력，동시취좌、우폐정맥혈진행혈기분석，취좌、우폐조직，관찰함수량급병이학개변。결과동물술후균존활，수착술후시간연장，공폐정맥혈양합지수（ PaO2／FiO2）하강화폐동맥압（ PAP）상승，여수체정상폐비교，차이유현저성（ P＜0.05）。수착시간적추이，이식폐조직출현수종、염성세포침윤、홍세포삼출，폐포벽증후명현，부분폐포강완전폐새，부분폐조직실변등변화，여수체폐조직비교，함수량증가현저（ P＜0.05）。결론위연구폐이식결혈재관주손상급면역배척반응연구궤제제공료이상적동물모형。
Objective To establish a porcine allogeneic left lung orthotopic transplantation model to closely simu-late human lung transplantation.Methods Twelve Huanjiang mini-pigs were used as donors and 12 Bama mini-pigs as recipients.The left lung orthotopic transplantation was completed by the left fourth intercostal thoracotomy.At 1 h, 2 h, 4 h, 6 h, 12 h after transplantation, the left and right pulmonary artery pressure were measured, the left and right pulmonary vein blood gas was analyzed, and samples of the left and right lung tissues were taken to determine the water content and for pathological examination.Results All animals survived, and the transplanted pulmonary vein blood PaO2/FiO2 and PAP were rised along with the prolonged postoperative time, compared with those of the recipient normal lung showing a signifi-cant difference (P<0.05).With the pass of time, there were increasing edema, inflammatory cell infiltration, RBC ooze, thickening of alveolar wall in the transplanted lung tissue, and some alveolar lumen occlusion and lung tissue consolidation. The water content of the transplanted lung tissue was increased significantly compared with that in the recipient lung tissue ( P<0.05 ) .Conclusions The established method in this study provides an ideal animal model for research on lung transplantation ischemia-reperfusion injury and immune rejection mechanism.