CAJ | 학술논문

目的：评估大骨节病患者软骨细胞的线粒体功能及其与年龄的关系。方法关节软骨细胞体外培养,分光光度法测定呼吸链复合体活性,化学发光法检测细胞内 ATP,流式细胞仪检测线粒体膜电位和细胞内活性氧含量,线性回归分析线粒体功能与年龄的相关性。结果大骨节病线粒体呼吸链复合体Ⅱ(P =0.001)、Ⅲ(P =0.005)和Ⅳ(P =0.005)活性降低,柠檬酸合酶活性升高(P =0.04),细胞内 ATP 含量减少(P =0.001),线粒体膜电位下降(P =0.001),细胞内活性氧含量增加(P <0.001)；线粒体功能与年龄在大骨节病和正常对照组均无显著相关性。结论大骨节病软骨细胞存在线粒体功能障碍,线粒体功能与年龄间无显著相关性。
목적：평고대골절병환자연골세포적선립체공능급기여년령적관계。방법관절연골세포체외배양,분광광도법측정호흡련복합체활성,화학발광법검측세포내 ATP,류식세포의검측선립체막전위화세포내활성양함량,선성회귀분석선립체공능여년령적상관성。결과대골절병선립체호흡련복합체Ⅱ(P =0.001)、Ⅲ(P =0.005)화Ⅳ(P =0.005)활성강저,저몽산합매활성승고(P =0.04),세포내 ATP 함량감소(P =0.001),선립체막전위하강(P =0.001),세포내활성양함량증가(P <0.001)；선립체공능여년령재대골절병화정상대조조균무현저상관성。결론대골절병연골세포존재선립체공능장애,선립체공능여년령간무현저상관성。
Objective To evaluate the mitochondrial function of chondrocytes in Kashin-Beck disease (KBD) patients and its correlation with age.Methods Mitochondrial function was evaluated by analyzing the activities of respiratory chain enzyme complexes and citrate synthase (CS),intracellular adenosine triphosphate (ATP)contents, changes in mitochondrial membrane potential (ΔΨm),as well as the intracellular reactive oxygen species (ROS) contents.Correlation of mitodhondrial function and age was analyzed with linear regression.Results Activities of complexes Ⅱ (P = 0.001 ),Ⅲ (P = 0.005 )and Ⅳ (P = 0.005 )were reduced in KBD articular chondrocytes compared with cells from normal controls.However,the mitochondrial mass was increased in KBD samples. Cultured KBD chondrocytes had reduced cellular ATP level (P = 0.001 )and mitochondrial membrane potential (P =0.001),but increased citrate synthase activity (P =0.04)and intracellular ROS level (P <0.001).We found no correlation between mitochondrial function and donor age either in normal or in KBD chondrocytes. Conclusion Mitochondrial function is altered in articular chondrocytes of KBD,and no correlation was found between mitochondrial function and donor age.