CAJ | 학술논문

目的：通过显微外科技术建立小鼠原位肺移植模型，为肺移植研究提供动物模型。方法采用C57BL／6小鼠作为供、受体，行同基因小鼠原位左肺移植，使用Cuff套管法进行气管及血管吻合。术后7、14、21、28 d取移植肺及原肺，行HE染色，评价肺移植后效果。结果学习曲线后，共30例小鼠移植，手术成功率89％，小鼠成活率100％。供体手术时间：（35.2±9.81）min，受体手术时间：（24.6±7.42） min，冷缺血时间是：（46.6± 8.92）min，热缺血时间是：（17.2±3.08）min。同基因移植物大体及病理无明显改变，病理显示与原肺无差别。结论本技术能够方便快捷建立小鼠肺移植模型，成功率高，可重复性强，符合原位肺移植临床生理，是研究肺移植发病机制和治疗的良好动物模型。
목적：통과현미외과기술건립소서원위폐이식모형，위폐이식연구제공동물모형。방법채용C57BL／6소서작위공、수체，행동기인소서원위좌폐이식，사용Cuff투관법진행기관급혈관문합。술후7、14、21、28 d취이식폐급원폐，행HE염색，평개폐이식후효과。결과학습곡선후，공30례소서이식，수술성공솔89％，소서성활솔100％。공체수술시간：（35.2±9.81）min，수체수술시간：（24.6±7.42） min，랭결혈시간시：（46.6± 8.92）min，열결혈시간시：（17.2±3.08）min。동기인이식물대체급병리무명현개변，병리현시여원폐무차별。결론본기술능구방편쾌첩건립소서폐이식모형，성공솔고，가중복성강，부합원위폐이식림상생리，시연구폐이식발병궤제화치료적량호동물모형。
Objective To investigate the lung transplantation by establishing an orthotopic mouse lung transplanta-tion model by microsurgery.Methods Thirty SPF male C57BL/6 mice served as both recipient and donor.Orthotopic lung transplantation models were established in the mice by three-cuff anastomotic technique.Native and transplanted lung tissue samples were taken at 7, 14, 21 and 28 days after transplantation for pathological evaluation of the outcomes using HE staining.Results After learning curve, 30 mice received transplantation.All mice survived and the success rate of lung transplantation was 89%.The donor operation time was 35.2 ±9.81 min and recipient operation time was 24.6 ± 7.42 min.Mean cold ischemia time was 46.6 ±8.92 min, and warm ischemia time was 17.2 ±3.08 min.No histological alterations were routinely detected in the isograft lungs at all times post-transplantation compared with the native lungs. Conclusions Mouse lung transplantation model can be successfully established by the established technique with a high success rate and excellent reproducibility, and is in accordance with the physiology of clinical orthotopic lung transplanta-tion.It is a good animal model for studying the pathogenesis and treatment of lung transplantation.