中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2015年
2期
178-181
,共4页
吴戈%肖蕾%张瑞丽%艾尼瓦尔·艾木都拉%张华%毛睿%程纬%王云莲%杨颖%包永星
吳戈%肖蕾%張瑞麗%艾尼瓦爾·艾木都拉%張華%毛睿%程緯%王雲蓮%楊穎%包永星
오과%초뢰%장서려%애니와이·애목도랍%장화%모예%정위%왕운련%양영%포영성
放射性肝损伤%放射性肝纤维化%TGF-β1,大鼠
放射性肝損傷%放射性肝纖維化%TGF-β1,大鼠
방사성간손상%방사성간섬유화%TGF-β1,대서
Radiation-induced liver injury%Radiation-induced hepatic fibrosis%Transforming growth factorβ1%Rats
目的：探讨急性放射性肝损伤大鼠模型的建立及TGF-β1动态变化的意义。方法选用雄性SD大鼠40只，随机分为模型组（30只）和对照组（10只）。除对照组外，模型组大鼠右半肝均接受单次6 MV X线25 Gy照射，接受照射第3、5、10天，应用HE染色、透射电镜下观察大鼠右肝病理学变化，血清学检测TGF-β1、肝功能相关指标（ ALT，AST，ALP），数据用SPSS 17.0统计软件进行分析。结果在照射第3、5、10天后，电镜下可观察肝脏早期病理学改变；TGF-β1的表达随时间延长而逐渐增加，模型组与对照组比较、不同时间段相比较，差异均有统计学意义（P＜0.05）；对照组和模型组的HE染色结果之间无明显差别；肝功能相关指标（ALT，AST，ALP）不同时间点比较无明显统计学意义（ P＞0.05）。结论电镜下观察肝脏病理学改变早于光镜下的变化，可用于早期放射性肝损伤的评价；TGF-β1较肝功能更加敏感地反映出照射后肝脏的损伤状态，可作为预测急性放射性肝损伤程度的血清细胞因子。
目的：探討急性放射性肝損傷大鼠模型的建立及TGF-β1動態變化的意義。方法選用雄性SD大鼠40隻，隨機分為模型組（30隻）和對照組（10隻）。除對照組外，模型組大鼠右半肝均接受單次6 MV X線25 Gy照射，接受照射第3、5、10天，應用HE染色、透射電鏡下觀察大鼠右肝病理學變化，血清學檢測TGF-β1、肝功能相關指標（ ALT，AST，ALP），數據用SPSS 17.0統計軟件進行分析。結果在照射第3、5、10天後，電鏡下可觀察肝髒早期病理學改變；TGF-β1的錶達隨時間延長而逐漸增加，模型組與對照組比較、不同時間段相比較，差異均有統計學意義（P＜0.05）；對照組和模型組的HE染色結果之間無明顯差彆；肝功能相關指標（ALT，AST，ALP）不同時間點比較無明顯統計學意義（ P＞0.05）。結論電鏡下觀察肝髒病理學改變早于光鏡下的變化，可用于早期放射性肝損傷的評價；TGF-β1較肝功能更加敏感地反映齣照射後肝髒的損傷狀態，可作為預測急性放射性肝損傷程度的血清細胞因子。
목적：탐토급성방사성간손상대서모형적건립급TGF-β1동태변화적의의。방법선용웅성SD대서40지，수궤분위모형조（30지）화대조조（10지）。제대조조외，모형조대서우반간균접수단차6 MV X선25 Gy조사，접수조사제3、5、10천，응용HE염색、투사전경하관찰대서우간병이학변화，혈청학검측TGF-β1、간공능상관지표（ ALT，AST，ALP），수거용SPSS 17.0통계연건진행분석。결과재조사제3、5、10천후，전경하가관찰간장조기병이학개변；TGF-β1적표체수시간연장이축점증가，모형조여대조조비교、불동시간단상비교，차이균유통계학의의（P＜0.05）；대조조화모형조적HE염색결과지간무명현차별；간공능상관지표（ALT，AST，ALP）불동시간점비교무명현통계학의의（ P＞0.05）。결론전경하관찰간장병이학개변조우광경하적변화，가용우조기방사성간손상적평개；TGF-β1교간공능경가민감지반영출조사후간장적손상상태，가작위예측급성방사성간손상정도적혈청세포인자。
Objective To explore the establishment of a rat model of acute radiation-induced liver injury and sig-nificance of the dynamic changes of TGF-β1 expression.Methods Forty healthy 6-week old male SD rats were randomly divided into model group (n=30) and control group (n=10).The right liver of rats in the model group was given a single dose of 25 Gy 6 MV X-ray irradiation.Histopathological examination using HE staining and transmission electron microsco-py were conducted to observe the liver pathological changes in rats at 3, 5, and 10 days after irradiation, serum TGF-β1 was detected, and relevant indicators of liver function ( ALT, AST, ALP) were determined.Statistical analysis was per-formed using SPSS 17.0 software.Results At 3, 5 and 10 days after irradiation, early pathological changes in the liver cells were observed by electron microscopy, the expression of TGF-β1 was gradually increased with the time prolongation, and significant differences were found between the model group and the control group at different time points (P<0.05). The light microscopic observation of liver tissues did not show significant differences between the control group and model group.The liver ALT, AST, ALP at different time points did not show significant differences between the two groups ( P>0.05).Conclusion Electron microscopy can be used to evaluate the early changes of radiation-induced liver injury, pri-or to the alterations visible by routine light microscopy.TGF-β1 can be used to predict the degree of radiation-induced liver injury, and may be used as a sensitive serum cytokine in predicting the degree of radiation-induced acute liver injury.
