CAJ | 학술논문

目的：观察养血消风饮对小鼠急、慢性皮炎-湿疹模型的抑制作用。方法：用二硝基氯苯分别建立小鼠耳廓慢性皮炎-湿疹模型及背部急性皮炎-湿疹模型。取60只小鼠，10只作为空白组，其余50只在耳部造成耳廓慢性皮炎-湿疹模型，分为模型组、养血消风饮低剂量组、养血消风饮中剂量组、养血消风饮高剂量组、氯雷他定组，每组10只；另取40只小鼠，10只作为空白组，其余30只在背部造成急性皮炎-湿疹模型，分为模型组、养血消风饮高剂量组、氯雷他定组，每组10只。采用灌胃给药的方法，养血消风饮低剂量组、中剂量组、高剂量组分别按每天成人等效剂量1倍、2倍、4倍给药（灌胃给药剂量分别为28．6 g·kg－1、57．2 g· kg－1、114．4 g · kg－1），氯雷他定组选用氯雷他定，按成人 4倍等效剂量给药（给药剂量为0．0052 g·kg－1），均调整灌胃给药量为0．4 mL。空白组、模型组均给予生理盐水0．4 mL灌胃。对比观察治疗前后动物模型耳肿胀度、脏器指数及组织结构的变化。结果：慢性皮炎-湿疹模型组小鼠的耳质量差值均明显高于空白组（P＜0．01），养血消风饮各剂量组和氯雷他定组的耳质量差与模型组比较，均明显减小（P＜0．01或P＜0．05）；慢性皮炎-湿疹模型组小鼠的脾指数、胸腺指数均明显升高（P＜0．01）；养血消风饮各剂量组和氯雷他定组均可显著下调急性、慢性皮炎-湿疹模型小鼠的脾指数及胸腺指数（P＜0．01）；养血消风饮可减轻二硝基氯苯造成急性皮炎-湿疹模型的表皮坏死、细胞内和细胞间水肿、真皮内炎性细胞浸润，从而减轻其对皮肤组织结构的破坏程度。结论：养血消风饮能减轻皮炎-湿疹模型小鼠耳廓的肿胀度，并能够抑制病变过程中免疫器官的肿大反应，下调胸腺指数和脾指数，从而减轻急性皮炎-湿疹小鼠病变局部组织损伤的程度。文献引用：芦源，关洪全．养血消风饮对小鼠皮炎－湿疹模型的影响[J]．中医学报，2015，30（5）：700－702．目的：觀察養血消風飲對小鼠急、慢性皮炎-濕疹模型的抑製作用。方法：用二硝基氯苯分彆建立小鼠耳廓慢性皮炎-濕疹模型及揹部急性皮炎-濕疹模型。取60隻小鼠，10隻作為空白組，其餘50隻在耳部造成耳廓慢性皮炎-濕疹模型，分為模型組、養血消風飲低劑量組、養血消風飲中劑量組、養血消風飲高劑量組、氯雷他定組，每組10隻；另取40隻小鼠，10隻作為空白組，其餘30隻在揹部造成急性皮炎-濕疹模型，分為模型組、養血消風飲高劑量組、氯雷他定組，每組10隻。採用灌胃給藥的方法，養血消風飲低劑量組、中劑量組、高劑量組分彆按每天成人等效劑量1倍、2倍、4倍給藥（灌胃給藥劑量分彆為28．6 g·kg－1、57．2 g· kg－1、114．4 g · kg－1），氯雷他定組選用氯雷他定，按成人 4倍等效劑量給藥（給藥劑量為0．0052 g·kg－1），均調整灌胃給藥量為0．4 mL。空白組、模型組均給予生理鹽水0．4 mL灌胃。對比觀察治療前後動物模型耳腫脹度、髒器指數及組織結構的變化。結果：慢性皮炎-濕疹模型組小鼠的耳質量差值均明顯高于空白組（P＜0．01），養血消風飲各劑量組和氯雷他定組的耳質量差與模型組比較，均明顯減小（P＜0．01或P＜0．05）；慢性皮炎-濕疹模型組小鼠的脾指數、胸腺指數均明顯升高（P＜0．01）；養血消風飲各劑量組和氯雷他定組均可顯著下調急性、慢性皮炎-濕疹模型小鼠的脾指數及胸腺指數（P＜0．01）；養血消風飲可減輕二硝基氯苯造成急性皮炎-濕疹模型的錶皮壞死、細胞內和細胞間水腫、真皮內炎性細胞浸潤，從而減輕其對皮膚組織結構的破壞程度。結論：養血消風飲能減輕皮炎-濕疹模型小鼠耳廓的腫脹度，併能夠抑製病變過程中免疫器官的腫大反應，下調胸腺指數和脾指數，從而減輕急性皮炎-濕疹小鼠病變跼部組織損傷的程度。文獻引用：蘆源，關洪全．養血消風飲對小鼠皮炎－濕疹模型的影響[J]．中醫學報，2015，30（5）：700－702．
목적：관찰양혈소풍음대소서급、만성피염-습진모형적억제작용。방법：용이초기록분분별건립소서이곽만성피염-습진모형급배부급성피염-습진모형。취60지소서，10지작위공백조，기여50지재이부조성이곽만성피염-습진모형，분위모형조、양혈소풍음저제량조、양혈소풍음중제량조、양혈소풍음고제량조、록뢰타정조，매조10지；령취40지소서，10지작위공백조，기여30지재배부조성급성피염-습진모형，분위모형조、양혈소풍음고제량조、록뢰타정조，매조10지。채용관위급약적방법，양혈소풍음저제량조、중제량조、고제량조분별안매천성인등효제량1배、2배、4배급약（관위급약제량분별위28．6 g·kg－1、57．2 g· kg－1、114．4 g · kg－1），록뢰타정조선용록뢰타정，안성인 4배등효제량급약（급약제량위0．0052 g·kg－1），균조정관위급약량위0．4 mL。공백조、모형조균급여생리염수0．4 mL관위。대비관찰치료전후동물모형이종창도、장기지수급조직결구적변화。결과：만성피염-습진모형조소서적이질량차치균명현고우공백조（P＜0．01），양혈소풍음각제량조화록뢰타정조적이질량차여모형조비교，균명현감소（P＜0．01혹P＜0．05）；만성피염-습진모형조소서적비지수、흉선지수균명현승고（P＜0．01）；양혈소풍음각제량조화록뢰타정조균가현저하조급성、만성피염-습진모형소서적비지수급흉선지수（P＜0．01）；양혈소풍음가감경이초기록분조성급성피염-습진모형적표피배사、세포내화세포간수종、진피내염성세포침윤，종이감경기대피부조직결구적파배정도。결론：양혈소풍음능감경피염-습진모형소서이곽적종창도，병능구억제병변과정중면역기관적종대반응，하조흉선지수화비지수，종이감경급성피염-습진소서병변국부조직손상적정도。문헌인용：호원，관홍전．양혈소풍음대소서피염－습진모형적영향[J]．중의학보，2015，30（5）：700－702．
Objective:To observe the inhibitory effect of Yangxue Xiaofeng Drink on mice acute and chronic dermatitis eczema model. Methods:To establish the chronic dermatitis eczema model of mouse ear and back of acute dermatitis eczema model with two nitro chlo-robenzene.From 60 mice,10 rats as the control group,the remaining 50 in the ear caused by chronic dermatitis eczema model of auri-cle,divided into model group,low dose group of Yangxue Xiaofeng Drink,middle dose group of Yangxue Xiaofeng Drink,and high dose group of Yangxue Xiaofeng Drink,loratadine group,with 10 rats in each group;another 40 small in 10 as the blank group,the remaining 30 on the back of the model of acute dermatitis and eczema,divided into the model group,high dose of Yangxue Xiaofeng drink group, loratadine group,with 10 rats in each group.By intragastric administration method of Yangxue Xiaofeng Decoction;low dose group,mid-dle dose group and high dose group respectively according to every adult equivalent dose of 1 times,2 times and 4 times of Administra-tion (gavage doses were 28.6 g·kg-1,57.2 g·kg-1,114.4 g·kg-1),loratadine group selection of loratadine,according to 4 times the adult equivalent dose (dose of 0.005 2 g·kg-1 ),adjusted by gavage dosage was 0.4 mL.The blank group and model group were given physiological saline 0.4 mL orally.In contrast to the changes observed in animal models of ear swelling degree,organ index and structure before and after the treatment.Results:The ear quality difference of chronic dermatitis eczema model group mice were signifi-cantly higher than those in the blank group (P<0.01),Chinese medicine dose group and Western medicine group in the ears of poor quality compared with the model group,were significantly decreased (P<0.01 or P<0.05 );chronic dermatitis eczema model group mice spleen index and thymus index were significantly increased(P<0.01);traditional Chinese medicine dose group and western med-icine group were significantly decreased in acute and chronic dermatitis eczema in mice spleen index and thymus index (P<0.01 );Yangxue Xiaofeng Decoction can reduce caused by acute dermatitis eczema model two nitrochlorobenzene epidermal necrosis,cellular and intercellular edema,inflammatory cell infiltration in the dermis,so as to reduce the degree of damage to the skin tissue structure. Conclusion:Yangxue Xiaofeng drink can reduce dermatitis eczema in mice auricle swelling,and swelling response suppression of im-mune organs of the pathological changes by the index of thymus and spleen,so as to reduce the acute dermatitis eczema lesion tissue damage in mice.