CAJ | 학술논문

目的：研究人慢性粒细胞白血病细胞株KCL22在NOD-SCID小鼠体内致白血病的能力，为慢性粒细胞白血病血液移植瘤模型鼠的建立奠定基础。方法取对数生长期的KCL22细胞2×107个，经尾静脉注射入NOD-SCID小鼠，对照组小鼠注射无菌PBS。观察小鼠一般情况，瑞氏染色监测血象和骨髓象变化，PCR检测骨髓细胞BCR-ABL基因转录水平，HE染色观察肝、脾组织肿瘤细胞浸润情况。结果实验组小鼠于注射细胞后约4周开始出现反应力下降、精神萎靡、股骨肌肿大、后肢骨节出血点等体征，外周血白细胞从第5周逐渐增多，计数较对照组显著升高（ P＜0.05），血涂片可见幼稚粒细胞，肝、脾、骨髓组织切片可见白血病细胞浸润，骨髓细胞高表达BCR-ABL融合基因，未经治疗存活约70天，较对照组显著缩短（ P＜0.05）。结论 KCL22细胞可成功构建NOD-SCID小鼠慢性粒细胞白血病移植瘤模型。
목적：연구인만성립세포백혈병세포주KCL22재NOD-SCID소서체내치백혈병적능력，위만성립세포백혈병혈액이식류모형서적건립전정기출。방법취대수생장기적KCL22세포2×107개，경미정맥주사입NOD-SCID소서，대조조소서주사무균PBS。관찰소서일반정황，서씨염색감측혈상화골수상변화，PCR검측골수세포BCR-ABL기인전록수평，HE염색관찰간、비조직종류세포침윤정황。결과실험조소서우주사세포후약4주개시출현반응력하강、정신위미、고골기종대、후지골절출혈점등체정，외주혈백세포종제5주축점증다，계수교대조조현저승고（ P＜0.05），혈도편가견유치립세포，간、비、골수조직절편가견백혈병세포침윤，골수세포고표체BCR-ABL융합기인，미경치료존활약70천，교대조조현저축단（ P＜0.05）。결론 KCL22세포가성공구건NOD-SCID소서만성립세포백혈병이식류모형。
Objective To investigate the potential of chronic myeloid leukemia ( CML) cell line KCL22 in indu-cing leukemia in NOD-SCID mice for setting up a basis for constructing a CML mouse transplantation tumor model. Methods 2 ×107 KCL22 cells in logarithmic growth phase were injected via the tail vein into experimental NOD-SCID mice whereas PBS was injected to the mice of control group.General condition of the mice of both groups was observed.Wright staining was used to observe the changes of blood and bone marrow smears.PCR was conducted to detect the transcription level of BCR-ABL, and histology with HE staining was used to evaluate the tumor cell invasion in the liver and spleen. Results Four weeks after the injection of KCL22 cells, the mice in experimental group showed physical signs of decreased reactivity, depression, swollen hindlimb muscles and petechia on the hindlimb femur.Peripheral white blood cells ( WBC) began to increase after 5 weeks, with a significantly increased quantity compared with the control group (P<0.05).Imma-ture granulocytes could be seen in blood and bone marrow smears, and tumor cell infiltration was found in the liver and spleen.BCR-ABL was highly expressed in bone marrow cells.Survival time of the experimental mice without therapy was 70 days, significantly shorter than that in the control group ( >90 days) (P<0.05).Conclusions A NOD-SCID mouse model of CML transplantation tumor is successfully established with leukemia KCL22 cells.