CAJ | 학술논문

目的：制备以生物纳米材料 pH 敏感嵌段共聚物聚乙二醇-聚天冬氨酸二异丙基氨基乙酯[PEG-PAsp (DIP)]传输 miR-21-inhibitor 聚合物囊泡，初步探讨 pH 敏感聚合物囊泡对前列腺癌细胞的毒性作用及传输效应。方法①通过静电作用，在室温条件下制备 PEG-PAsp (DIP)传输 miR-21-inhibitor 聚合物囊泡；②通过凝胶阻滞实验检测不同浓度下纳米聚合物囊泡(NPs)的复合情况；③通过 CCK-8实验检测不同浓度下 pH 敏感 NPs 对前列腺癌细胞 PC-3的毒性作用；④通过激光共聚焦实验，观察 PEG-PAsp (DIP)/ miR-21-inhibitor 纳米聚合物囊泡进入 PC-3细胞的传输效应。结果实验结果表明： N/ P 为20 PEG-PAsp (DIP)与 miR-21-inhibitor 已完全复合，粒径较小具有一定的正电荷，且对 PC-3细胞毒性较小；通过激光共聚焦实验，成功构建了纳米聚合物囊泡传输进入 PC-3前列腺癌细胞方法。结论成功制备了纳米聚合物囊泡，并初步探讨了纳米聚合物囊泡的表征、体外 PC-3细胞毒性试验与纳米聚合物囊泡结合 miRNA传输于 PC-3细胞的激光共聚焦实验，为下一步阐明反义 miR-21寡核苷酸诱导前列腺癌细胞凋亡的作用和分子机制打好基础。
목적：제비이생물납미재료 pH 민감감단공취물취을이순-취천동안산이이병기안기을지[PEG-PAsp (DIP)]전수 miR-21-inhibitor 취합물낭포，초보탐토 pH 민감취합물낭포대전렬선암세포적독성작용급전수효응。방법①통과정전작용，재실온조건하제비 PEG-PAsp (DIP)전수 miR-21-inhibitor 취합물낭포；②통과응효조체실험검측불동농도하납미취합물낭포(NPs)적복합정황；③통과 CCK-8실험검측불동농도하 pH 민감 NPs 대전렬선암세포 PC-3적독성작용；④통과격광공취초실험，관찰 PEG-PAsp (DIP)/ miR-21-inhibitor 납미취합물낭포진입 PC-3세포적전수효응。결과실험결과표명： N/ P 위20 PEG-PAsp (DIP)여 miR-21-inhibitor 이완전복합，립경교소구유일정적정전하，차대 PC-3세포독성교소；통과격광공취초실험，성공구건료납미취합물낭포전수진입 PC-3전렬선암세포방법。결론성공제비료납미취합물낭포，병초보탐토료납미취합물낭포적표정、체외 PC-3세포독성시험여납미취합물낭포결합 miRNA전수우 PC-3세포적격광공취초실험，위하일보천명반의 miR-21과핵감산유도전렬선암세포조망적작용화분자궤제타호기출。
Objective To prepare pH sensitive PEG-PAsp (DIP) / miR-21-inhibitor polymer nanoparticles (NPs) and examine the toxicity of the polymer NPs and their transmission effects on prostate cancer cells. Methods ① The PEG-PAsp ( DIP) / miR-21-inhibitor polymer NPs were prepared through electrostatic interaction at the room temperature. ② The gel retardation assay was used to detect the composition of polymer NPs of different concentrations. ③ CCK-8 cytotoxicity as-say was employed to test the toxic effects of different concentrations of NPs on prostate cancer PC-3 cells. ④ The transmission effect of PEG-PAsp (DIP) / miR-21-inhibitor polymer NPs was detected on PC-3 cells. Results When N/ P was 20, PEG-PAsp (DIP) was completely bound to miR-21-inhibitor. The particle diameter was small and had certain positive charge and lower toxicity to PC-3 cells. Confocal laser experiment showed that the constructed polymer NPs were successfully trans-ferred into PC-3 cells. Conclusion The PEG-PAsp (DIP) / miR-21-inhibitor polymer NPs were successfully constructed, and the NPs characterization and in vitro cytotoxicity to PC-3 cell and the transmission effects in PC-3 cells were examined. Our study lays a good foundation for further re-searching the role and molecular mechanism of miR-21-inhibitor-induced apoptosis in prostate cancer cells.