CAJ | 학술논문

目的探讨复合氨基酸联合维生素 B6治疗四氧化二氮吸入中毒肺水肿小鼠的疗效。方法实验动物为 ICR 小鼠24只，体重23～25 g，随机分为对照组、中毒组和治疗组等三组（8只）。将动物置于120 L 染毒柜制作 N2 O4吸入中毒致肺损伤动物模型，于48 h 采用眼球摘除法取血，分离血清－70℃保存，ELISA 法测定 ANP、SP-D 含量。结果对照组小鼠血清 ANP 和 SP-D 含量均值分别为[（221．74±30．82）mg／L、（24．09±2．79）mg／L]；中毒组分别为[（313．46±31．58）mg／L、（20．40±4．27）mg／L]；药物干预组分别为（262．03±26．50、23．20±2．84）。中毒组与对照组之间 ANP 和 SP-D水平差异有统计学意义（tANP ＝7．85，tSP-D ＝6．89，P ＜0．05），治疗组与中毒组之间 ANP 和 SP-D 水平差异有统计学意义（tANP ＝4．52，tSP-D ＝3．67，P ＜0．05）。结论采用丰诺安联用维生素 B6能明显改善 N2 O4吸入中毒动物血清 ANP 和 SP-D 代谢水平，促进病情转归，对于救治此类氮氧化物中毒患者具有一定参考价值。
목적탐토복합안기산연합유생소 B6치료사양화이담흡입중독폐수종소서적료효。방법실험동물위 ICR 소서24지，체중23～25 g，수궤분위대조조、중독조화치료조등삼조（8지）。장동물치우120 L 염독거제작 N2 O4흡입중독치폐손상동물모형，우48 h 채용안구적제법취혈，분리혈청－70℃보존，ELISA 법측정 ANP、SP-D 함량。결과대조조소서혈청 ANP 화 SP-D 함량균치분별위[（221．74±30．82）mg／L、（24．09±2．79）mg／L]；중독조분별위[（313．46±31．58）mg／L、（20．40±4．27）mg／L]；약물간예조분별위（262．03±26．50、23．20±2．84）。중독조여대조조지간 ANP 화 SP-D수평차이유통계학의의（tANP ＝7．85，tSP-D ＝6．89，P ＜0．05），치료조여중독조지간 ANP 화 SP-D 수평차이유통계학의의（tANP ＝4．52，tSP-D ＝3．67，P ＜0．05）。결론채용봉낙안련용유생소 B6능명현개선 N2 O4흡입중독동물혈청 ANP 화 SP-D 대사수평，촉진병정전귀，대우구치차류담양화물중독환자구유일정삼고개치。
Objective To explore the diagnosis and treatment compound amino acid and vitamin B6 nitrogen tetroxide inhalation poisoning the curative effect of pulmonary edema.Methods ICR mice 24, weight 23-25 g (n =8),were randomly divided into three groups:control group,poisoned group,and treatment group.The mice were put in the 120 L exposed cabinet to inhalate nitrogen oxides as environmental exposure mode.Bloods were collected by the eye-excised method at 48 h time point,the serum was separated by -70℃ preservation,and the ELISA was used to detect the contents of ANP and SP-D.Results Serum ANP and SP-D were (221.74 ±30.82)mg/L and (24.09 ±2.79)mg/L in control group,(313.46 ± 31.58)mg/Land (20.40 ±4.27)mg/L in the poisoned group,and (262.03 ±26.50)mg/L and (23.20 ± 2.84)mg/L in the treatment group,respectively.We used SPSS20.0 software to perform single factor analysis of variance,and P <0.05 was set as statistical significance.The difference was statistically significant between the poisoned group and the control group in the ANP and SP-D levels (tANP =7.85, tSP-D =6.89,P <0.05 ),and the same to that between the poisoned group and the treatment group in the ANP and SP-D levels (tANP =4.52,tSP-D =3.67,P <0.05 ),but no significant difference existed in the level of TGF-β1.Conclusions It leads to impairment of pulmonary edema in short time exposure of nitrogen oxides.To detect the level of ANP and SP-D could be helpful to study the pulmonary edema. Compound amino acid and vitamin B6 could obviously improve the level of serum ANP and SP-D in the animals poisoned by N2 O4 ,and disease outcome,which have a certain reference value in the treatment of such patients.