CAJ | 학술논문

目的：研究西格列汀对2型糖尿病大鼠血清及不同组织视黄醇结合蛋白4（RBP4）表达水平的影响。方法将35只SD大鼠随机分为两组，即对照组（NC，n＝10）和高脂组（HF，n＝25）。12周后，HF组大鼠腹腔一次性注射链脲佐菌素35 mg／kg，成功建立2型糖尿病模型大鼠20只，并随机分为糖尿病对照组（DM组，n＝10）和西格列汀干预组（SP组，n＝10）。分别测定干预前后体质量（BW）、空腹血糖（FBG）、三酰甘油（TG）、胆固醇（TCH）、低密度脂蛋白（LDL-C）、空腹胰岛素（FINS）及血清RBP4水平，并计算胰岛素抵抗指数（HOMA-IR）和胰岛β细胞功能指数（HOMA-β）。Western Blot法检测大鼠不同组织RBP4蛋白表达水平。结果①与NC组相比，DM组、SP组BW、FBG、TG、TCH、LDL-C、FINS、HOMA-IR、RBP4升高，HOMA-β降低，差异有统计学意义（P＜0．05）；西格列汀干预后，SP组较DM组FBG、TG、TCH、LDL-C、FINS、HOMA-IR、RBP4降低，HOMA-β升高，差异有统计学意义（P＜0．05）。②与NC组相比，DM组附睾脂肪、肌肉组织RBP4蛋白表达水平显著增加（P＜0．05）；与DM组相比，SP组附睾脂肪、肌肉组织RBP4蛋白表达水平显著降低（P＜0．05）。结论2型糖尿病大鼠血清RBP4水平升高，附睾脂肪、肌肉组织RBP4蛋白表达水平增高。西格列汀能显著降低2型糖尿病大鼠血清RBP4水平及附睾脂肪、肌肉组织中RBP4蛋白表达水平。
목적：연구서격렬정대2형당뇨병대서혈청급불동조직시황순결합단백4（RBP4）표체수평적영향。방법장35지SD대서수궤분위량조，즉대조조（NC，n＝10）화고지조（HF，n＝25）。12주후，HF조대서복강일차성주사련뇨좌균소35 mg／kg，성공건립2형당뇨병모형대서20지，병수궤분위당뇨병대조조（DM조，n＝10）화서격렬정간예조（SP조，n＝10）。분별측정간예전후체질량（BW）、공복혈당（FBG）、삼선감유（TG）、담고순（TCH）、저밀도지단백（LDL-C）、공복이도소（FINS）급혈청RBP4수평，병계산이도소저항지수（HOMA-IR）화이도β세포공능지수（HOMA-β）。Western Blot법검측대서불동조직RBP4단백표체수평。결과①여NC조상비，DM조、SP조BW、FBG、TG、TCH、LDL-C、FINS、HOMA-IR、RBP4승고，HOMA-β강저，차이유통계학의의（P＜0．05）；서격렬정간예후，SP조교DM조FBG、TG、TCH、LDL-C、FINS、HOMA-IR、RBP4강저，HOMA-β승고，차이유통계학의의（P＜0．05）。②여NC조상비，DM조부고지방、기육조직RBP4단백표체수평현저증가（P＜0．05）；여DM조상비，SP조부고지방、기육조직RBP4단백표체수평현저강저（P＜0．05）。결론2형당뇨병대서혈청RBP4수평승고，부고지방、기육조직RBP4단백표체수평증고。서격렬정능현저강저2형당뇨병대서혈청RBP4수평급부고지방、기육조직중RBP4단백표체수평。
Objective To evaluate the effects of sitagliptin on metabolic parameters,serum retinol binding protein 4 (RBP4)lev-els,liver,epididymal adipose tissue and skeletal muscle RBP4 expression levels in a rat model of type 2 diabetes mellitus (T2DM). Meth-ods Tirty-five Sprague Dawley rats were randomly divided into normal diet group (NC group)of 10 rats and high fat diet group (HF group) of the remaining 25. After being fed on a high fat diet for 12 weeks,the HF group was given the peritoneal injection of a low dose of strepto-zotocin 35 mg/kg,and a rat model of T2DM (n=20)was established and randomly divided into two groups:diabetic control group (DM group,n=10),sitagliptin group (SP group,n=10). Body weight (BW),fasting blood glucose (FBG),triglyceride (TG),total choles-terol (TCH),low-density lipoprotein (LDL-C),fasting insulin (FINS),serum RBP4 were measured respectively before and after the 6 weeks of treatment,and homeostasis model assessment-insulin resistance index (HOMA-IR),homeostasis model assessment β-cell function index (HOMA-β)were calculated. The liver,epididymal adipose tissue and skeletal muscle RBP4 protein expression levels were detected by Western blot. Results ①The levels of BW,FBG,TG,TCH,LDL-C,FINS,HOMA-IR and serum RBP4 in DM group and SP group in-creased while the levels of HOMA-β decreased compared with those of NC group with a statistical significance (P<0. 05 ). After treatment with sitagliptin,the levels of FBG,TG,TCH,LDL-C,FINS,HOMA-IR and serum RBP4 of SP group decreased while the levels of HOMA-β increased compared with those of DM group with a statistical significance (P<0. 05 ). ② Epididymal adipose tissue and skeletal muscle RBP4 protein expression levels in DM group increased compared with those in NC group,and epididymal adipose tissue and skeletal muscle RBP4 protein expression levels in SP group decreased compared with those in DM group. Conclusion Serum,epididymal adipose tissue and skeletal muscle RBP4 protein levels are elevated in a rat model of T2DM. Sitagliptin can lower serum,epididymal adipose tissue and skeletal muscle RBP4 protein levels in a rat model of T2DM.