CAJ | 학술논문

目的：研究速效牙痛宁酊中芫花酯甲的限量测定方法。方法采用 Agilent HC －C18柱（4．6mm ×250mm，5μm），以乙腈∶0．01％醋酸溶液为流动相，采用梯度洗脱，流速1．0mL／min，柱温35℃，检测波长238nm，芫花酯甲在7．50－150．0μg／mL 浓度范围内有良好线性关系（r ＝0．9999），平均加样回收率为98．9％，RSD 为1．09％。结果经HPLC 测定其芫花酯甲的含量为5．02μg／mL，对10批制剂进行检测其含量均小于6．0μg／mL。结论本方法灵敏度高、准确、重复性好、专属性强，可用于测定速效牙痛宁酊中芫花酯甲的限量。
목적：연구속효아통저정중원화지갑적한량측정방법。방법채용 Agilent HC －C18주（4．6mm ×250mm，5μm），이을정∶0．01％작산용액위류동상，채용제도세탈，류속1．0mL／min，주온35℃，검측파장238nm，원화지갑재7．50－150．0μg／mL 농도범위내유량호선성관계（r ＝0．9999），평균가양회수솔위98．9％，RSD 위1．09％。결과경HPLC 측정기원화지갑적함량위5．02μg／mL，대10비제제진행검측기함량균소우6．0μg／mL。결론본방법령민도고、준학、중복성호、전속성강，가용우측정속효아통저정중원화지갑적한량。
Abstracts:Objective To establish a method for determination of yuanhuacin in Suxiao Yatongning Tinct.Method The HPLC system consisted of the Agilent HC -C18 (4.6mm ×250mm, 5μm)column, the mobile phase consisted of acetonitrile and 0.01% HAc, gradient e-lution flow rate was 1.0mL/min, the column temperature was 35℃, the UV detector was set at 238nm.The linear response range were 7.50-150.0μg/mL(r =0.9999).The average recovery of yuanhuacin was 98.4% and RSD was 1.37%.Results The determination of yuan-huacin were less than 6.0μg/mL within 10 batches of preparation and determination of the content was 5.02μg/mL.Conclusions The method was high sensitivity, accurate, repeatable and high secificity.It can be an important method to control the standard of Suxiao Ya-tongning Tinct.