CAJ | 학술논문

目的：使用气相色谱－质谱联用仪，采用选择监测离子法测定洁尔阴洗液中蛇床子素的含量。方法DB －5MS（30mm ×0．32 mm ×0．25μm）石英毛细管柱为色谱柱，大黄酚为内标，样品经过乙酸乙酯直接提取，选择离子监测法定量分析，监测离子为 m／z ＝244。结果蛇床子素在1－100μg／mL（r ＝0．9986）范围内线性良好；平均回收率为98．3％，检测限为1．0 ng／mL。结论本方法简便、快速、灵敏度高、结果准确可靠，可以用于洁尔阴洗液中蛇床子素含量的测定。
목적：사용기상색보－질보련용의，채용선택감측리자법측정길이음세액중사상자소적함량。방법DB －5MS（30mm ×0．32 mm ×0．25μm）석영모세관주위색보주，대황분위내표，양품경과을산을지직접제취，선택리자감측법정량분석，감측리자위 m／z ＝244。결과사상자소재1－100μg／mL（r ＝0．9986）범위내선성량호；평균회수솔위98．3％，검측한위1．0 ng／mL。결론본방법간편、쾌속、령민도고、결과준학가고，가이용우길이음세액중사상자소함량적측정。
Abstracts:Objective To develop a new method of GC -MS assay for the determination of osthole in Jieeryin Lotion.Method Osthole was analyzed quantitatively on the DB -5MS quartz capillary chromatographic column by elective ion monitoring and selective ion was m/z =244.Chry-sophanol was an internal standard.The sample was extracted by ultrasound in acetic ether.Results There was a good linear relationship in the range of 1 -100μg/mL(r =0.9986) for osthole.The average recovery was 98.3% and the detection limit was 1.0 ng/mL.Conclution The method is convenient, of good sensibility and accuracy, it can be used to determinate the content of osthole in Jieeryin Lotion.