CAJ | 학술논문

目的：对合欢皮水提取物分离纯化后各部位进行急性毒性观察与评价，为后期抗焦虑药效实验给药剂量的确定提供依据。方法采用急性毒理学研究的方法探究合欢皮水提取物分离纯化后各部位对小鼠的毒性剂量；应用最大耐受量法测定各个部位的最大给药量或最大耐受量。结果按含生药量计算，合欢皮水提取物由 D101大孔树脂柱分离得到的以下部位最大耐受量分别为：水部位（D －H20）1343．20g／kg；40％乙醇部位（D －40EtOH）1178．58g／kg；95％乙醇部位（D －95EtOH）99．94g／kg；其中40％乙醇部位经过 SKP 反相树脂柱分离得到的以下部位最大耐受量或最大给药量分别为：40％甲醇部位（S －40MeOH）531．15g／kg；75％甲醇部位（S －75MeOH）2898．40 g／kg；90％甲醇部位（S－90MeOH）47506．52g／kg。结论合欢皮水提取物经分离纯化后所得到的6个化学部位，最大耐受量或最大给药量均不同。由 D101大孔树脂柱分离所得3个部位中，95％乙醇部位的最大耐受剂量明显低于水部位及40％乙醇部位，其毒性最大；40％乙醇部位经过 SKP 反相树脂柱分离得到的3个部位中，40％甲醇部位最大耐受量明显低于75％甲醇部位及95％甲醇部位。40％乙醇部位的毒性成分可能主要转移至40％甲醇部位。
목적：대합환피수제취물분리순화후각부위진행급성독성관찰여평개，위후기항초필약효실험급약제량적학정제공의거。방법채용급성독이학연구적방법탐구합환피수제취물분리순화후각부위대소서적독성제량；응용최대내수량법측정각개부위적최대급약량혹최대내수량。결과안함생약량계산，합환피수제취물유 D101대공수지주분리득도적이하부위최대내수량분별위：수부위（D －H20）1343．20g／kg；40％을순부위（D －40EtOH）1178．58g／kg；95％을순부위（D －95EtOH）99．94g／kg；기중40％을순부위경과 SKP 반상수지주분리득도적이하부위최대내수량혹최대급약량분별위：40％갑순부위（S －40MeOH）531．15g／kg；75％갑순부위（S －75MeOH）2898．40 g／kg；90％갑순부위（S－90MeOH）47506．52g／kg。결론합환피수제취물경분리순화후소득도적6개화학부위，최대내수량혹최대급약량균불동。유 D101대공수지주분리소득3개부위중，95％을순부위적최대내수제량명현저우수부위급40％을순부위，기독성최대；40％을순부위경과 SKP 반상수지주분리득도적3개부위중，40％갑순부위최대내수량명현저우75％갑순부위급95％갑순부위。40％을순부위적독성성분가능주요전이지40％갑순부위。
Abstracts:Objective To observe and evaluate the acute toxicity of different parts separated and purified from the water extract of Albi-zza julibrissin bark.It can also give evidence for the dose of the following anxiolytic -like test.Method Using classic researching methods of acute toxicology to determine the toxic dose of different parts separated and purified from the water extract of Albizza julibrissin bark.The maximum dose or maximum tolerated dose of each parts was measured by the maximum tolerated dose(MTD)researching method.Results The maximum tolerated doses or maximum dose of the different parts from the water extract of Albizza julibrissin bark eluted after the D101 macroporous resin are as the following: the water part (D -H20) is 1343.20 g/kg; the 40% ethanol part(D -40EtOH) is 1178.58 g/kg;the 95% ethanol part(D -95EtOH) is 99.94 g/kg; The maximum tolerated doses of the different parts from the 40% ethanol part eluted af-ter SKP RP resin are as the following : the 40% methanol part(S -40MeOH) is 531.15 g/kg; the 95% methanol part(S -75MeOH) is 2898.40 g/kg;the 90% methanol part(S -90MeOH) is 47506.52 g/kg.Conclusion The maximum dose or maximum tolerated dose of the six parts separated and purified from the water extract of Albizza julibrissin bark are different.In the three parts eluted after the D101 macro-porous resin,the maximum tolerated dose of 95% ethanol part is lower than the 40% ethanol part or water part obviously and it shows the lar-gest toxicity;however,in the three parts from the 40% ethanol part eluted after SKP RP resin,the maximum tolerated dose of 40% methanol part is lower than the 75% methanol part or 95% methanol part obviously.The toxic components might be transferred from the 40% ethanol part to the 40% methanol part.