辽宁农业科学
遼寧農業科學
료녕농업과학
LIAONING AGRICULTURAL SCIENCES
2015年
2期
23-27
,共5页
熊剑锐%何俊蓉%蒋彧%李萍
熊劍銳%何俊蓉%蔣彧%李萍
웅검예%하준용%장욱%리평
中国兰%春剑‘隆昌素’%叶色突变体%光合特性
中國蘭%春劍‘隆昌素’%葉色突變體%光閤特性
중국란%춘검‘륭창소’%협색돌변체%광합특성
Chinese orchid%Cymbidium ‘longchangsu’%The leaf color mutant%Phtosynthetic characteristics
以中国兰春剑‘隆昌素’正常绿色组培苗为对照,对它的叶色黄化突变体的光合参数、光响应曲线及模拟参数、荧光动力学参数、CO2响应曲线进行了测定,试验结果表明突变体的净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)均低于对照,胞间二氧化碳浓度(Ci)高于对照。叶绿素荧光动力学分析结果表明,叶色黄化突变体的最大荧光产量(Fm)较低,其 Fv /Fm、ΦPSⅡ和 qP 均高于突变亲本,这表明叶色黄化突变体具有较高的光能捕获效率和转换效率。叶色黄化突变体的暗呼吸速率、表观量子效率和羧化效率都低于突变亲本,光呼吸速率也是低于突变亲本的。
以中國蘭春劍‘隆昌素’正常綠色組培苗為對照,對它的葉色黃化突變體的光閤參數、光響應麯線及模擬參數、熒光動力學參數、CO2響應麯線進行瞭測定,試驗結果錶明突變體的淨光閤速率(Pn)、氣孔導度(Gs)、蒸騰速率(Tr)均低于對照,胞間二氧化碳濃度(Ci)高于對照。葉綠素熒光動力學分析結果錶明,葉色黃化突變體的最大熒光產量(Fm)較低,其 Fv /Fm、ΦPSⅡ和 qP 均高于突變親本,這錶明葉色黃化突變體具有較高的光能捕穫效率和轉換效率。葉色黃化突變體的暗呼吸速率、錶觀量子效率和羧化效率都低于突變親本,光呼吸速率也是低于突變親本的。
이중국란춘검‘륭창소’정상록색조배묘위대조,대타적협색황화돌변체적광합삼수、광향응곡선급모의삼수、형광동역학삼수、CO2향응곡선진행료측정,시험결과표명돌변체적정광합속솔(Pn)、기공도도(Gs)、증등속솔(Tr)균저우대조,포간이양화탄농도(Ci)고우대조。협록소형광동역학분석결과표명,협색황화돌변체적최대형광산량(Fm)교저,기 Fv /Fm、ΦPSⅡ화 qP 균고우돌변친본,저표명협색황화돌변체구유교고적광능포획효솔화전환효솔。협색황화돌변체적암호흡속솔、표관양자효솔화최화효솔도저우돌변친본,광호흡속솔야시저우돌변친본적。
The normal green Chinese orchid Cymbidium ‘longcangsu’in tissue culture was served as control group,we measured the photosynthetic parameters,light response curve and the simulation parameters,fluores-cence induction kinetic parameters,CO2 -response curve of the aurea mutant,the result showed that the net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)were lower in the mutants, with higher in-tercellular CO2 concentration (Ci).Chlorophyll fluorescence showed that Fm of the aurea mu-tant were lower than control.Compared with control,the aurea mutants displayed higher optional maximal pho-tochemical efficiency of PS II (Fv /Fm),actual photo-chemical efficiency of PSⅡ(ΦPSⅡ),and photochemi-cal quenching (qP),suggesting that the mutants have higher light energy cap-ture and conversion efficiencies as well as the electron transport efficiency.Also the aurea mutant have lower dark respiration rate,apparent quantum yield,cargoxylation efficiency and photorespiratory rate than control.