肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2015年
3期
145-148,152
,共5页
刘尽国%丁闻洁%叶敏%赵兰香%秦钢%张杰
劉儘國%丁聞潔%葉敏%趙蘭香%秦鋼%張傑
류진국%정문길%협민%조란향%진강%장걸
肺肿瘤%腺癌%钙结合蛋白%表皮生长因子受体%磷脂酰肌醇3激酶%免疫组织化学%预后
肺腫瘤%腺癌%鈣結閤蛋白%錶皮生長因子受體%燐脂酰肌醇3激酶%免疫組織化學%預後
폐종류%선암%개결합단백%표피생장인자수체%린지선기순3격매%면역조직화학%예후
Lung neoplasms%Adenocarcinoma%Calcium-binding protein%Epidermal growth factor receptor%Phosphatidylinositol 3-kinase%Immunohistochemistry%Prognosis
目的 探讨S100钙结合蛋白A4(S100A4)、表皮生长因子受体(EGFR)及磷脂酰肌醇3激酶(PI3K)在肺腺癌组织中的表达、临床病理意义及其相互关系.方法 应用免疫组织化学EnVision二步法检测84例肺腺癌组织、30例正常肺组织中S100A4、EGFR及PI3K蛋白的表达,并分析三者与肺腺癌临床病理因素、术后5年生存率的关系及三者间表达的相关性.结果 84例肺腺癌组织中S100A4、EGFR及PI3K的阳性表达率分别为69.0％(58/84)、64.3％(54/84)、52.4％(44/84),高于正常肺组织的6.7％(2/30)、16.7 ％(5/30)、13.3 ％(4/30)(均P＜0.01).84例肺腺癌组织中S100A4、EGFR及PI3K蛋白的表达与肿瘤分化程度、淋巴结转移、临床分期及5年生存有关(P＜0.05),与患者性别、年龄及肿块大小无关(P＞0.05).肺腺癌组织中S100A4与EGFR、PI3K的表达呈正相关(均P＜ 0.01),EGFR与PI3K的表达呈正相关(P＜0.01).结论 S100A4、EGFR、PI3K与肺腺癌的发生、发展、转移及预后相关.S100A4蛋白可作为判断肺腺癌生物学行为及预测转移趋势的重要指标之一.S100A4与EGFR、PI3K的表达相关.
目的 探討S100鈣結閤蛋白A4(S100A4)、錶皮生長因子受體(EGFR)及燐脂酰肌醇3激酶(PI3K)在肺腺癌組織中的錶達、臨床病理意義及其相互關繫.方法 應用免疫組織化學EnVision二步法檢測84例肺腺癌組織、30例正常肺組織中S100A4、EGFR及PI3K蛋白的錶達,併分析三者與肺腺癌臨床病理因素、術後5年生存率的關繫及三者間錶達的相關性.結果 84例肺腺癌組織中S100A4、EGFR及PI3K的暘性錶達率分彆為69.0％(58/84)、64.3％(54/84)、52.4％(44/84),高于正常肺組織的6.7％(2/30)、16.7 ％(5/30)、13.3 ％(4/30)(均P＜0.01).84例肺腺癌組織中S100A4、EGFR及PI3K蛋白的錶達與腫瘤分化程度、淋巴結轉移、臨床分期及5年生存有關(P＜0.05),與患者性彆、年齡及腫塊大小無關(P＞0.05).肺腺癌組織中S100A4與EGFR、PI3K的錶達呈正相關(均P＜ 0.01),EGFR與PI3K的錶達呈正相關(P＜0.01).結論 S100A4、EGFR、PI3K與肺腺癌的髮生、髮展、轉移及預後相關.S100A4蛋白可作為判斷肺腺癌生物學行為及預測轉移趨勢的重要指標之一.S100A4與EGFR、PI3K的錶達相關.
목적 탐토S100개결합단백A4(S100A4)、표피생장인자수체(EGFR)급린지선기순3격매(PI3K)재폐선암조직중적표체、림상병리의의급기상호관계.방법 응용면역조직화학EnVision이보법검측84례폐선암조직、30례정상폐조직중S100A4、EGFR급PI3K단백적표체,병분석삼자여폐선암림상병리인소、술후5년생존솔적관계급삼자간표체적상관성.결과 84례폐선암조직중S100A4、EGFR급PI3K적양성표체솔분별위69.0％(58/84)、64.3％(54/84)、52.4％(44/84),고우정상폐조직적6.7％(2/30)、16.7 ％(5/30)、13.3 ％(4/30)(균P＜0.01).84례폐선암조직중S100A4、EGFR급PI3K단백적표체여종류분화정도、림파결전이、림상분기급5년생존유관(P＜0.05),여환자성별、년령급종괴대소무관(P＞0.05).폐선암조직중S100A4여EGFR、PI3K적표체정정상관(균P＜ 0.01),EGFR여PI3K적표체정정상관(P＜0.01).결론 S100A4、EGFR、PI3K여폐선암적발생、발전、전이급예후상관.S100A4단백가작위판단폐선암생물학행위급예측전이추세적중요지표지일.S100A4여EGFR、PI3K적표체상관.
Objective To investigate the expression,clinicopathological significance and correlation of S100A4,EGFR and PI3K in lung adenocarcinoma.Methods Immunohistochemical method (EnVision two steps) was used to detect the expression of S100A4,EGFR and PI3K proteins in 84 lung adenocarcinoma tissue samples and 30 normal lung tissue samples.The relationship of S100A4,EGFR and PI3K expression with clinicopathologic factors,post-operative five-year survival and the correlations among the three proteins were analyzed.Results The positive expression rates of S100A4,EGFR and PI3Kin lung adenocarcinoma tissues were higher than those in normal tissues,respectively [(69.0 ％,58/84) vs (6.7 ％,2/30),(64.3 ％,54/84) vs (16.7 ％,5/30),(52.4 ％,44/84) vs (13.3 ％,4/30),P ＜ 0.01].The expression of S100A4,EGFR and PI3K proteins were positively correlated with the differentiated degree,lymph node metastasis,clinical stages,and five-year survival (P ＜ 0.05),but not correlated with other clinicopathologic factors (P ＞ 0.05).The expression of S100A4 was positively correlated with EGFR and PI3K in lung adenocarcinoma (P ＜ 0.01),and the expression of EGFR was positively correlated with PI3K (P＜ 0.01).Conclusions S100A4,EGFR and PI3K were closely related with the occurrence,development,metastasis and prognosis of lung adenocarcinoma.S100A4 might be an important marker in estimating biological behavior and metastasis tendence of lung adenocarcinoma.S100A4 may be correlated with EGFR and PI3K.
