CAJ | 학술논문

目的探讨miR-128在急性淋巴细胞白血病(ALL)中的表达及其意义.方法采用实时荧光定量聚合酶链反应法对62例ALL患者和20例骨髓正常患者骨髓单个核细胞miR-128的相对表达量进行检测,并与患者的临床资料进行相关性分析.结果 miR-128在ALL患者中的相对表达量高于健康对照组(P＜0.05),但其在急性B淋巴细胞白血病(B-ALL)及急性T淋巴细胞白血病(T-ALL)中的表达水平差异无统计学意义(P＞0.05),miR-128的表达与是否存在bcr-abl融合基因及其mRNA的表达水平均无相关性(均P＞ 0.05).结论 miR-128在ALL患者中表达上调,可作为临床诊断ALL的潜在分子标志物,bcr-abl融合基因的表达对miR-128在ALL中的表达无明显影响.
목적 탐토miR-128재급성림파세포백혈병(ALL)중적표체급기의의.방법 채용실시형광정량취합매련반응법대62례ALL환자화20례골수정상환자골수단개핵세포miR-128적상대표체량진행검측,병여환자적림상자료진행상관성분석.결과 miR-128재ALL환자중적상대표체량고우건강대조조(P＜0.05),단기재급성B림파세포백혈병(B-ALL)급급성T림파세포백혈병(T-ALL)중적표체수평차이무통계학의의(P＞0.05),miR-128적표체여시부존재bcr-abl융합기인급기mRNA적표체수평균무상관성(균P＞ 0.05).결론 miR-128재ALL환자중표체상조,가작위림상진단ALL적잠재분자표지물,bcr-abl융합기인적표체대miR-128재ALL중적표체무명현영향.
Objective To investigate the expression and clinical significance of miR-128 in acute lymphoblastic leukemia (ALL).Methods The relative expression levels of miR-128 from bone marrow cells were detected by real time PCR in 62 patients with ALL and 20 normal subjects,and the correlations between miR-128 expression and its clinical characteristics were analyzed.Results The relative expression level of miR-128 in ALL was higher than that in normal subjects (P ＜ 0.05),but there was no statistical difference in miR-128 expression levels between B-ALL and T-ALL patients (P ＞ 0.05),and no significant correlation was found between miR-128 expression level and bcr-abl positive rates or bcr-abl mRNA level in ALL (P ＞ 0.05).Conclusion miR-128 is up-regulated in ALL patients,which may provide a novel ALL diagnostic marker.There was no obvious influence of bcr-abl fusion gene on miR-128 expression in ALL patients.