CAJ | 학술논문

目的：探讨血清中N端前脑钠肽（ NT－proBNP）的最佳保存条件，为检测试剂盒提供性能稳定的检测参考品。方法：采用ELISA定量检测不同保存液中NT－proBNP含量的变化，单因素分析基础缓冲体系、防腐剂Proclin300及抗生素等成分对NT－proBNP保存的影响，再通过正交实验进行各因素的优化组合，确定最佳的NT－proBNP保存体系。结果：单因素确定NT－proBNP保存基础缓冲体系为0．02 mol／L pH7．2的PBS缓冲液；添加防腐剂Proclin300可使NT－proBNP在37℃中的保存时间延长1 d；选择联合添加青霉素、链霉素比各自单独添加延长NT－proBNP保存时间1 d。通过正交实验确定NT－proBNP保存液成分为0．02 mol／L pH7．2的PBS基础缓冲液配制的20％的小牛血清、1／1000的Proclin300、120 U／ml青霉素与80 U／ml链霉素，以此保存液保存NT－proBNP参考品7 d后标定的定值样本结果显示：37℃中与4℃中标定值相比仅降低了1．3％。结论：优化后的NT－proBNP血清保存液能在37℃条件下保存NT－proBNP参考品7 d。
목적：탐토혈청중N단전뇌납태（ NT－proBNP）적최가보존조건，위검측시제합제공성능은정적검측삼고품。방법：채용ELISA정량검측불동보존액중NT－proBNP함량적변화，단인소분석기출완충체계、방부제Proclin300급항생소등성분대NT－proBNP보존적영향，재통과정교실험진행각인소적우화조합，학정최가적NT－proBNP보존체계。결과：단인소학정NT－proBNP보존기출완충체계위0．02 mol／L pH7．2적PBS완충액；첨가방부제Proclin300가사NT－proBNP재37℃중적보존시간연장1 d；선택연합첨가청매소、련매소비각자단독첨가연장NT－proBNP보존시간1 d。통과정교실험학정NT－proBNP보존액성분위0．02 mol／L pH7．2적PBS기출완충액배제적20％적소우혈청、1／1000적Proclin300、120 U／ml청매소여80 U／ml련매소，이차보존액보존NT－proBNP삼고품7 d후표정적정치양본결과현시：37℃중여4℃중표정치상비부강저료1．3％。결론：우화후적NT－proBNP혈청보존액능재37℃조건하보존NT－proBNP삼고품7 d。
Objective:To investigate the best preservation conditions of N-terminal pro-brain natriuretic peptide( NT-proBNP) and provide detection references with stable performance for detection kits.Methods: ELISA was used to quantitatively detect the changes in NT-proBNP contents in various preservation solutions.The effects of basic buffer system, preservative Proclin300 and antibiotics on the preservation of NT-proBNP were analyzed using univariate analysis.The combination of various factors was then optimized using orthogonal experiments, to identify the best preservation system for NT-proBNP.Results: The univariate analysis determined that the basic buffer system for NT-proBNP was 0.02 mol/L phosphate buffered saline(PBS) at pH7.2,the addition of pre-servative Proclin300 could extend the preservation time of NT-proBNP at 37℃ by one day, the combined addition of penicillin and streptomycin prolonged the preservation time of NT-proBNP by one day compared with individually adding penicillin or streptomycin.The orthogonal experiments identified a preservation solution for NT-proBNP as 20%calf serum,1/1 000 Proclin300,120 U/ml penicillin and 80 U/ml streptomycin in a basic buffer system of 0.02 mol/L PBS at pH7.2.This solution was used to preserve an NT-proBNP reference sample at 37℃.Seven days later,the calibrated fixed-value of the sample at 37℃was only 1.3%lower than that at 4℃.Conclusion:Optimized NT-proBNP serum preservation solution could preserve NT-proBNP standard sample at 37℃ for seven days.