中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
4期
514-516
,共3页
李鑫%丁镌%侯玲%王鑫%颜廷宇%李继红%张春燕%赵素珍%刘颖%刘杰
李鑫%丁鐫%侯玲%王鑫%顏廷宇%李繼紅%張春燕%趙素珍%劉穎%劉傑
리흠%정전%후령%왕흠%안정우%리계홍%장춘연%조소진%류영%류걸
分型%新等位基因%SBT%三维结构模拟
分型%新等位基因%SBT%三維結構模擬
분형%신등위기인%SBT%삼유결구모의
Typing%A novel allele%Sequencing Based Typing%Modeling of three-dimensional structure
目的：确认HLA新等位基因HLA－A?01∶130并分析其有异常反应格局的核苷酸序列。方法：应用DNA测序分型技术（ PCR－SBT）进行HLA分型，对可疑新等位基因采用单链测序基因分型技术直接测定基因序列，分析其与同源性最高的HLA等位基因序列的差异。最后，经Swiss－Model对HLA分子进行三维结构模拟。结果：新基因与所有已知的HLA－A等位基因序列均不相同，与HLA－A?01∶66基因序列相比在第3外显子368位碱基由A→G，导致第99位密码子改变，酪氨酸→半胱氨酸。替代氨基酸位于抗原肽结合区β片层上。结论：发现一个新的HLA－A等位基因，现已被世界卫生组织HLA因子命名委员会正式命名为HLA－A?01∶130。
目的：確認HLA新等位基因HLA－A?01∶130併分析其有異常反應格跼的覈苷痠序列。方法：應用DNA測序分型技術（ PCR－SBT）進行HLA分型，對可疑新等位基因採用單鏈測序基因分型技術直接測定基因序列，分析其與同源性最高的HLA等位基因序列的差異。最後，經Swiss－Model對HLA分子進行三維結構模擬。結果：新基因與所有已知的HLA－A等位基因序列均不相同，與HLA－A?01∶66基因序列相比在第3外顯子368位堿基由A→G，導緻第99位密碼子改變，酪氨痠→半胱氨痠。替代氨基痠位于抗原肽結閤區β片層上。結論：髮現一箇新的HLA－A等位基因，現已被世界衛生組織HLA因子命名委員會正式命名為HLA－A?01∶130。
목적：학인HLA신등위기인HLA－A?01∶130병분석기유이상반응격국적핵감산서렬。방법：응용DNA측서분형기술（ PCR－SBT）진행HLA분형，대가의신등위기인채용단련측서기인분형기술직접측정기인서렬，분석기여동원성최고적HLA등위기인서렬적차이。최후，경Swiss－Model대HLA분자진행삼유결구모의。결과：신기인여소유이지적HLA－A등위기인서렬균불상동，여HLA－A?01∶66기인서렬상비재제3외현자368위감기유A→G，도치제99위밀마자개변，락안산→반광안산。체대안기산위우항원태결합구β편층상。결론：발현일개신적HLA－A등위기인，현이피세계위생조직HLA인자명명위원회정식명명위HLA－A?01∶130。
Objective:To confirm the novel allele HLA-A*01∶130 and analyzed the nucleotide sequence of the abnormal reaction pattern.Methods: The HLA typing of sample DNA was performed by PCR-SBT.The ambiguous novel HLA allele was confirmed with single stranded SBT method,then DNA sequencing was performed to identify the difference between the novel allele and HLA-A?01:66 allele.Finally, it was modeled by Swiss-Model to three-dimensional structure of HLA Molecule.Results: The novel allele was not the same with all known HLA-A allele sequence.After analysis,there was one nucleotide differed from the A?01:66 at position 368 where A→G( codon 99 TAT→TGT) resulting in a coding change,99 Tyr was changed to Cys.The amino acid substitution at residue 99 the HLA polypeptide was located in a beta-sheet of antigenic peptide-binding region.Conclusion: The allele is a novel allele that has now been officially named as HLA-A*01∶130 by the World Health Organization( WHO) HLA Nomenclature Committee.
