临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2015年
8期
675-677
,共3页
妊娠%米非司酮%胚胎生长%转化生长因子β 肿瘤坏死因子α
妊娠%米非司酮%胚胎生長%轉化生長因子β 腫瘤壞死因子α
임신%미비사동%배태생장%전화생장인자β 종류배사인자α
Gestation%Mifepristone%Embryonic growth%Transforming growth factor beta%Tumor necrosis factor α
目的：探讨米非司酮对胚胎生长、早孕蜕膜和绒毛中转化生长因子β（ TGF-β）以及血清肿瘤坏死因子α（ TNF-α）的影响。方法临床纳入自愿进行人工流产的妊娠妇女90例，根据人工流产术前处理方案的不同分为研究组与对照组。研究组人工流产术前给予100 mg米非司酮口服，对照组不给于米非司酮口服。使用反转录-聚合酶链反应（RT-PCR）检测两组早孕蜕膜和绒毛中TGF-β1以及受体TGF-β1R基因的表达情况；使用放射免疫法检测研究组服药前后血清TNF-α的水平。结果研究组蜕膜中TGF-β1以及受体TGF-β1R基因表达明显高于对照组（1.66±0.61 vs.1.00±0.46；1.54±0.50 vs.0.89±0.30），差异均有显著性（ P ﹤0.05）；两组绒毛中TGF-β1以及受体TGF-β1R基因表达未见明显差异（ P ﹥0.05）。研究组服用米非司酮后血清TNF-α明显高于服药前（0.87±0.14μg/L vs.0.55±0.10μg/L），差异有显著性（ P ﹤0.05）。结论米非司酮能够明显组阻止胚胎的生长、发育，其能够通过下调蜕膜中TGF-β1以及受体TGF-β1R的转录，提升血清TNF-α的水平，从母-胎界面局部至外周，促使免疫刺激以及抑制失调，造成母体对胚胎排斥而不利于妊娠的维持。
目的：探討米非司酮對胚胎生長、早孕蛻膜和絨毛中轉化生長因子β（ TGF-β）以及血清腫瘤壞死因子α（ TNF-α）的影響。方法臨床納入自願進行人工流產的妊娠婦女90例，根據人工流產術前處理方案的不同分為研究組與對照組。研究組人工流產術前給予100 mg米非司酮口服，對照組不給于米非司酮口服。使用反轉錄-聚閤酶鏈反應（RT-PCR）檢測兩組早孕蛻膜和絨毛中TGF-β1以及受體TGF-β1R基因的錶達情況；使用放射免疫法檢測研究組服藥前後血清TNF-α的水平。結果研究組蛻膜中TGF-β1以及受體TGF-β1R基因錶達明顯高于對照組（1.66±0.61 vs.1.00±0.46；1.54±0.50 vs.0.89±0.30），差異均有顯著性（ P ﹤0.05）；兩組絨毛中TGF-β1以及受體TGF-β1R基因錶達未見明顯差異（ P ﹥0.05）。研究組服用米非司酮後血清TNF-α明顯高于服藥前（0.87±0.14μg/L vs.0.55±0.10μg/L），差異有顯著性（ P ﹤0.05）。結論米非司酮能夠明顯組阻止胚胎的生長、髮育，其能夠通過下調蛻膜中TGF-β1以及受體TGF-β1R的轉錄，提升血清TNF-α的水平，從母-胎界麵跼部至外週，促使免疫刺激以及抑製失調，造成母體對胚胎排斥而不利于妊娠的維持。
목적：탐토미비사동대배태생장、조잉세막화융모중전화생장인자β（ TGF-β）이급혈청종류배사인자α（ TNF-α）적영향。방법림상납입자원진행인공유산적임신부녀90례，근거인공유산술전처리방안적불동분위연구조여대조조。연구조인공유산술전급여100 mg미비사동구복，대조조불급우미비사동구복。사용반전록-취합매련반응（RT-PCR）검측량조조잉세막화융모중TGF-β1이급수체TGF-β1R기인적표체정황；사용방사면역법검측연구조복약전후혈청TNF-α적수평。결과연구조세막중TGF-β1이급수체TGF-β1R기인표체명현고우대조조（1.66±0.61 vs.1.00±0.46；1.54±0.50 vs.0.89±0.30），차이균유현저성（ P ﹤0.05）；량조융모중TGF-β1이급수체TGF-β1R기인표체미견명현차이（ P ﹥0.05）。연구조복용미비사동후혈청TNF-α명현고우복약전（0.87±0.14μg/L vs.0.55±0.10μg/L），차이유현저성（ P ﹤0.05）。결론미비사동능구명현조조지배태적생장、발육，기능구통과하조세막중TGF-β1이급수체TGF-β1R적전록，제승혈청TNF-α적수평，종모-태계면국부지외주，촉사면역자격이급억제실조，조성모체대배태배척이불리우임신적유지。
Objective To explore the effect of mifepristone on embryonic growth and transforming growth factor β( TGF-β)in decidua and villi,and serum tumor necrosis factorα( TNF-α). Methods Ninety pregnant women with voluntary artificial abortion were divided into study group and control group according to the difference in induced abortion before treatment scheme. Pregnant women in study group were admin-istrated with 100 mg mifepristone before induced abortion,and pregnant women in control group had not received mifepristone. The expression of TGF-β1 and TGF-β1 R in decidus and villus of 2 groups was examined by reverse transcription polymerase chain reaction,and serum level of TNF-α was examined by radioimmunoassay. Results The gene expressions of TGF-β1 in decidua and TGF-β1R in study group were 1. 66 ± 0. 61 and 1. 54 ± 0. 50 respectively,and they were 1 ± 0. 46 and 0. 89 ± 0. 30 respectively in control group,and the difference was significant( P﹤0. 05);the difference in expression of TGF-β1 and TGF-β1R in villi between these two groups was not significant( P ﹥0. 05). The serum level of TNF-αin study group before taking mifepristone was 0. 55 ± 0. 10 μg/L,and serum level of TNF-αafter taking the drug was 0. 87 ± 0. 14 μg/L,and. their difference was significant( P ﹤0. 05). Conclusion Mifepristone can obviously prevent the growth of embryo,it can raise the serum level of TNF-αthrough down-regulation of decidual TGF-β1 and TGF-β1 R transcription,prompting the immune stimulation and inhibition of disorders from maternal and fetal interface from local to peripheral,resulting in the body of mother and not conductive to the mainte-nance of fetal rejection of pregnancy.
