中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
4期
537-540
,共4页
Th17细胞%调节性T细胞%卵巢癌%白细胞介素17
Th17細胞%調節性T細胞%卵巢癌%白細胞介素17
Th17세포%조절성T세포%란소암%백세포개소17
Th17 cell%Regulatory T cell%Ovarian cancer%Interleukin-17
目的：检测卵巢癌患者外周血中辅助性T细胞17（T help cell 17，Th17）／调节性T细胞（regulatory T cell， Treg）的水平并探讨其临床意义。方法：选取55例卵巢癌和60例健康对照者为研究对象，采用细胞内染色流式细胞术（ Flow cytometry，FCM）、实时定量PCR（real time PCR）、酶联免疫吸附法（Enzyme linked immunosorbent assay，ELISA）法检测外周血Th17／Treg细胞百分率、核转录因子retinoid－related orphan receptor gamma－t（RORγt）／foxhead winged－helix box protein 3（Foxp3） mRNA的表达以及血浆中白细胞介素17（Interleukin－17，IL－17）／转化生长因子－β1（Transforming growth factor－β1，TGF－β1）的水平。结果：卵巢癌患者外周血中CD4＋IL－17＋／CD4＋T细胞百分率、RORγt mRNA及IL－17水平与正常对照组相比明显升高，差异有统计学意义（ P＜0．05）。与正常对照组相比，卵巢癌患者外周血中CD4＋Foxp3＋Treg／CD4＋T细胞百分率、Foxp3 mRNA及TGF－β1水平明显升高，差异有统计学意义（P＜0．05）。结论：卵巢癌患者外周血中Th17／Treg细胞失衡，Th17／Treg细胞可能参与卵巢癌的发病过程。
目的：檢測卵巢癌患者外週血中輔助性T細胞17（T help cell 17，Th17）／調節性T細胞（regulatory T cell， Treg）的水平併探討其臨床意義。方法：選取55例卵巢癌和60例健康對照者為研究對象，採用細胞內染色流式細胞術（ Flow cytometry，FCM）、實時定量PCR（real time PCR）、酶聯免疫吸附法（Enzyme linked immunosorbent assay，ELISA）法檢測外週血Th17／Treg細胞百分率、覈轉錄因子retinoid－related orphan receptor gamma－t（RORγt）／foxhead winged－helix box protein 3（Foxp3） mRNA的錶達以及血漿中白細胞介素17（Interleukin－17，IL－17）／轉化生長因子－β1（Transforming growth factor－β1，TGF－β1）的水平。結果：卵巢癌患者外週血中CD4＋IL－17＋／CD4＋T細胞百分率、RORγt mRNA及IL－17水平與正常對照組相比明顯升高，差異有統計學意義（ P＜0．05）。與正常對照組相比，卵巢癌患者外週血中CD4＋Foxp3＋Treg／CD4＋T細胞百分率、Foxp3 mRNA及TGF－β1水平明顯升高，差異有統計學意義（P＜0．05）。結論：卵巢癌患者外週血中Th17／Treg細胞失衡，Th17／Treg細胞可能參與卵巢癌的髮病過程。
목적：검측란소암환자외주혈중보조성T세포17（T help cell 17，Th17）／조절성T세포（regulatory T cell， Treg）적수평병탐토기림상의의。방법：선취55례란소암화60례건강대조자위연구대상，채용세포내염색류식세포술（ Flow cytometry，FCM）、실시정량PCR（real time PCR）、매련면역흡부법（Enzyme linked immunosorbent assay，ELISA）법검측외주혈Th17／Treg세포백분솔、핵전록인자retinoid－related orphan receptor gamma－t（RORγt）／foxhead winged－helix box protein 3（Foxp3） mRNA적표체이급혈장중백세포개소17（Interleukin－17，IL－17）／전화생장인자－β1（Transforming growth factor－β1，TGF－β1）적수평。결과：란소암환자외주혈중CD4＋IL－17＋／CD4＋T세포백분솔、RORγt mRNA급IL－17수평여정상대조조상비명현승고，차이유통계학의의（ P＜0．05）。여정상대조조상비，란소암환자외주혈중CD4＋Foxp3＋Treg／CD4＋T세포백분솔、Foxp3 mRNA급TGF－β1수평명현승고，차이유통계학의의（P＜0．05）。결론：란소암환자외주혈중Th17／Treg세포실형，Th17／Treg세포가능삼여란소암적발병과정。
Objective:To detect the levels of T help cell 17 (Th17)/regulatory T cell (Treg) in the peripheral blood of patients with ovarian cancer and explore their clinical significances.Methods:55 patients with ovarian cancer and 60 normal controls were enrolled.The percentage of Th17/Treg cells were detected by flow cytometry (FCM).The expression of retinoid-related orphan receptor gamma-t ( RORγt)/foxhead winged-helix box protein 3 ( Foxp3) mRNA was measured by real time PCR and the plasma levels of IL-17/TGF-β1 were detected by enzyme linked immunosorbent assay (ELISA).Results: The percentage of CD4+IL-17+/CD4+T cells,the mRNA expression of RORγt and the plasma levels of IL-17 increased significantly in patients with ovarian cancer than in normal controls (P<0.05).Compared with normal controls,the percentage of CD4+Foxp3+Treg/CD4+T cells,the mRNA expression of Foxp3 and the plasma levels of TGF-β1 increased significantly in patients with ovarian cancer ( P<0.05).Conclusion:The imbalance of Th17/Treg cells may exist in the peripheral blood of patients with ovarian cancer.Th17/Treg cells may participate the pathology of ovarian cancer.
