CAJ | 학술논문

目的：了解多药耐药肺炎克雷伯菌（MDRKP）中5种毒力基因存在情况，为临床治疗提供参考依据。方法收集2008年8月－2010年5月杭州市和湖州市6所医院共47株MDRKP ，采用PCR及序列分析方法检测5种毒力基因（arr、magA、rmpA、hofA和ompT）。结果47株MDRKP中检测到 arr和magA 两种毒力基因，检出率分别为29．8％和17．0％；6所医院 A、B、C、D、E、F的MDRKP分离株中毒力基因 arr的检出阳性率分别为26．7％、81．8％、0、14．3％、0、0，6所医院A、B、C、D、E、F的MDRKP分离株中毒力基因 magA的检出阳性率分别为0、0、0、0、0、88．9％。结论 MDRKP中毒力基因 arr和magA 具有一定的携带率，在MDRKP中检出毒力基因 arr和magA 为国内首次报道。
목적：료해다약내약폐염극뢰백균（MDRKP）중5충독력기인존재정황，위림상치료제공삼고의거。방법수집2008년8월－2010년5월항주시화호주시6소의원공47주MDRKP ，채용PCR급서렬분석방법검측5충독력기인（arr、magA、rmpA、hofA화ompT）。결과47주MDRKP중검측도 arr화magA 량충독력기인，검출솔분별위29．8％화17．0％；6소의원 A、B、C、D、E、F적MDRKP분리주중독력기인 arr적검출양성솔분별위26．7％、81．8％、0、14．3％、0、0，6소의원A、B、C、D、E、F적MDRKP분리주중독력기인 magA적검출양성솔분별위0、0、0、0、0、88．9％。결론 MDRKP중독력기인 arr화magA 구유일정적휴대솔，재MDRKP중검출독력기인 arr화magA 위국내수차보도。
OBJECTIVE To investigate the distribution of 5 kinds of virulence genes in 47 strains of multidrug‐resistant K lebsiella pneumoniae (MDRKP) ,so as to provide reference for clinical treatment .METHODS Totally 47 strains of MDRKP from Aug .2008 to May 2010 were collected from 6 hospitals in Hangzhou and Huzhou in Zhejiang Province in China .Then ,5 kinds of virulence genes(arr ,magA ,rmpA ,hofA ,ompT) were analyzed by PCR .RESULTS In 47 strains of MDRKP ,2 kinds of virulence genes (arr ,magA)were detected ,and the de‐tection rates were 29 .8% (14/47)and 17 .0% (8/47) .The detection rates of arr in hospitals A ,B ,C ,D ,E ,F were 26 .7% ,81 .8% ,0 ,14 .3% ,0 ,0 ,respectively .The detection rates of magA in hospitals A ,B ,C ,D ,E , F were 0 ,0 ,0 ,0 ,0 ,88 .9% , respectively .CONCLUSION Virulence genes (arr , magA )were positive in MDRKP .And this is the first report that virulence genes(arr ,magA)were positive in MDRKP .