CAJ | 학술논문

目的：研究液基薄层细胞学（TCT ）、荧光定量聚合酶链反应技术（FQPCR）、P16蛋白联合检测在宫颈癌早期诊断中的价值，为宫颈癌的早期防治提供参考。方法对2012年10月－2013年10月1786例宫颈异常女性进行TCT、FQPCR检查，监测人乳头状瘤病毒（HPV）感染情况，检测P16蛋白表达，采用SPSS 17．0进行统计分析。结果 1786例患者中检测 HPV阳性198例，阳性率为11．09％；其中高危型 HPV占38．38％、低危型占61．62％；P16蛋白随着CINⅡ级、CINⅢ级、SCC变化表达率逐渐升高，比较差异有统计学意义（ P＜0．05）。结论宫颈异常患者 HPV阳性检出率高，P16蛋白表达率随着宫颈病变的加重而提升，FQPCR、TCT、P16联合检查有助于宫颈癌及其癌前病变的筛查。
목적：연구액기박층세포학（TCT ）、형광정량취합매련반응기술（FQPCR）、P16단백연합검측재궁경암조기진단중적개치，위궁경암적조기방치제공삼고。방법대2012년10월－2013년10월1786례궁경이상녀성진행TCT、FQPCR검사，감측인유두상류병독（HPV）감염정황，검측P16단백표체，채용SPSS 17．0진행통계분석。결과 1786례환자중검측 HPV양성198례，양성솔위11．09％；기중고위형 HPV점38．38％、저위형점61．62％；P16단백수착CINⅡ급、CINⅢ급、SCC변화표체솔축점승고，비교차이유통계학의의（ P＜0．05）。결론궁경이상환자 HPV양성검출솔고，P16단백표체솔수착궁경병변적가중이제승，FQPCR、TCT、P16연합검사유조우궁경암급기암전병변적사사。
OBJECTIVE To study TCT ,FQPCR ,P16 protein combined detection in early diagnosis of cervical canc‐er ,so as to provide a reference for early prevention and treatment of cervical cancer .METHODS Totally 1786 fe‐male patients with cervical abnormalities during Oct .2012 to Oct .2013 were performed with thinprep cytology test (TCT ) and fluorescent quantitation polymerase chain reaction (FQPCR) test to detect human papillomavirus (HPV) infection and expression of P16 protein . The software SPSS 17 .0 was used for statistical analysis . RESULTS Totally 198 cases were detected positive for HPV accounting for 11 .09% ,including high‐risk HPV (38 .38% ) and low‐risk HPV (61 .62% ) .The expression of P16 was significantly increased along with CIN Ⅱ , CIN Ⅲ and SCC (P<0 .05) .CONCLUSION Patients with cervical abnormalities have higher HPV‐positive detec‐tion rate .P16 protein expression increased with aggravation of cervical lesions .FQPCR ,TCT ,P16 combined de‐tection contributes to screening of precancerous lesions and cervical cancer .