中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
11期
1699-1706
,共8页
刘振东%蔡绪%王魁向%张远军%刘志涛
劉振東%蔡緒%王魁嚮%張遠軍%劉誌濤
류진동%채서%왕괴향%장원군%류지도
组织构建%骨组织构建%骨折愈合%2型糖尿病%Osx%Dlx5%骨痂%湖南省自然科学基金
組織構建%骨組織構建%骨摺愈閤%2型糖尿病%Osx%Dlx5%骨痂%湖南省自然科學基金
조직구건%골조직구건%골절유합%2형당뇨병%Osx%Dlx5%골가%호남성자연과학기금
Diabetes Melitus,Type 2%Bony Calus%Fracture Healing
背景:研究表明Osx、Dlx5等基因的表达水平与骨折愈合障碍可能存在一定关系。目的:分析2型糖尿病大鼠牵引骨痂中Osx、Dlx5表达变化与骨折愈合障碍的关系。方法:选择8周龄SD大鼠32只,随机分为2组,实验组(18只)高糖高脂饲料喂养8周,链脲佐菌素腹腔注射制作2型糖尿病大鼠模型;对照组喂普通大鼠饲料予以同样剂量柠檬酸腹腔注射。2组大鼠同时建立骨折牵引模型,定期延长外固定架,牵引模型建立后15 d处死大鼠并收集血液标本检测生化指标,X射线观察骨痂生长情况,取左胫骨骨痂组织进行组织学观察,检测骨痂组织中Dlx5、Osx蛋白及相关基因的表达。结果与结论:X射线摄片结果:实验组牵引骨痂较对照组明显减少,骨痂组织苏木精-伊红染色显示:实验组较对照组的微骨柱明显减少;初始基质前沿浅染。而骨痂的组织学定量分析则显示:实验组新生骨痂的形成面积较对照组减少而骨折近端脂肪细胞数量增多(P <0.01)。QPCR检测则显示:实验组较对照组骨痂组织中Osx表达降低(P <0.05),Dlx5表达降低(P <0.05)。结果提示,2型糖尿病大鼠骨折愈合较正常大鼠明显减慢,可能与2型糖尿病Osx、Dlx5表达降低具有相关性。
揹景:研究錶明Osx、Dlx5等基因的錶達水平與骨摺愈閤障礙可能存在一定關繫。目的:分析2型糖尿病大鼠牽引骨痂中Osx、Dlx5錶達變化與骨摺愈閤障礙的關繫。方法:選擇8週齡SD大鼠32隻,隨機分為2組,實驗組(18隻)高糖高脂飼料餵養8週,鏈脲佐菌素腹腔註射製作2型糖尿病大鼠模型;對照組餵普通大鼠飼料予以同樣劑量檸檬痠腹腔註射。2組大鼠同時建立骨摺牽引模型,定期延長外固定架,牽引模型建立後15 d處死大鼠併收集血液標本檢測生化指標,X射線觀察骨痂生長情況,取左脛骨骨痂組織進行組織學觀察,檢測骨痂組織中Dlx5、Osx蛋白及相關基因的錶達。結果與結論:X射線攝片結果:實驗組牽引骨痂較對照組明顯減少,骨痂組織囌木精-伊紅染色顯示:實驗組較對照組的微骨柱明顯減少;初始基質前沿淺染。而骨痂的組織學定量分析則顯示:實驗組新生骨痂的形成麵積較對照組減少而骨摺近耑脂肪細胞數量增多(P <0.01)。QPCR檢測則顯示:實驗組較對照組骨痂組織中Osx錶達降低(P <0.05),Dlx5錶達降低(P <0.05)。結果提示,2型糖尿病大鼠骨摺愈閤較正常大鼠明顯減慢,可能與2型糖尿病Osx、Dlx5錶達降低具有相關性。
배경:연구표명Osx、Dlx5등기인적표체수평여골절유합장애가능존재일정관계。목적:분석2형당뇨병대서견인골가중Osx、Dlx5표체변화여골절유합장애적관계。방법:선택8주령SD대서32지,수궤분위2조,실험조(18지)고당고지사료위양8주,련뇨좌균소복강주사제작2형당뇨병대서모형;대조조위보통대서사료여이동양제량저몽산복강주사。2조대서동시건립골절견인모형,정기연장외고정가,견인모형건립후15 d처사대서병수집혈액표본검측생화지표,X사선관찰골가생장정황,취좌경골골가조직진행조직학관찰,검측골가조직중Dlx5、Osx단백급상관기인적표체。결과여결론:X사선섭편결과:실험조견인골가교대조조명현감소,골가조직소목정-이홍염색현시:실험조교대조조적미골주명현감소;초시기질전연천염。이골가적조직학정량분석칙현시:실험조신생골가적형성면적교대조조감소이골절근단지방세포수량증다(P <0.01)。QPCR검측칙현시:실험조교대조조골가조직중Osx표체강저(P <0.05),Dlx5표체강저(P <0.05)。결과제시,2형당뇨병대서골절유합교정상대서명현감만,가능여2형당뇨병Osx、Dlx5표체강저구유상관성。
BACKGROUND:Experiments have shown that the expression levels of Osx and Dlx5 may have some relationships with fracture healing disorder. OBJECTIVE:To probe into the relationship between the change of expression of Osx and Dlx5 in the calus of type 2 diabetes rats and fracture healing disorder. METHODS:We selected 32 Sprague-Dawley rats with proper weight in 8 weeks age. They were randomly assigned into two groups: the experimental group (n=18) was fed with high fat and sugar diet for 8 weeks, and then received intraperitoneal injection of streptozotocin to induce diabetic models; the control group was fed with normal diet and received intraperitoneal injection of the same dose of citric acid. Two weeks later, al the rats were used to establish left tibia traction fracture models with orthopedic external fixator. We extended the external fixator at a proper length per day. The animals were sacrificed at 15 days after establishment of traction fracture models. The blood specimens were evaluated for biochemical detection. X-ray was used to observe the growth of calus. The calus tissues from the left tibia were taken for histological observation and the expression of Dlx5 and Osx in the calus was detected. RESULTS AND CONCLUSION:X-ray results showed that the amount of calus tissues in the experimental group was significantly reduced. The hematoxylin-eosin staining of the calus tissue revealed that the microcolumn formation in the experimental group was significantly reduced compared with the control group; the area of primary matrixfront was lightly colored. The calus histological quantitative analysis showed that in the experimental group the area of new calus decreased and more adipose cels presented in the proximal fracture
<br> end compared with the control group (P < 0.01). QPCR displayed that the expression of Osx and Dlx5 was lower in the experimental group than the control group (P < 0.05). These findings indicate that the poorer fracture healing in type 2 diabetes than the normal rats may be associated with the decrease of Osx and Dlx5 expression.