解放军医学院学报
解放軍醫學院學報
해방군의학원학보
Academic Journal of Chinese Pla Medical School
2015年
4期
371-373,387
,共4页
徐小龙%苟文龙%王程%袁雪凌%王玉%彭江%卢世璧%郭全义%汪爱媛%许文静%孟昊业%刘舒云
徐小龍%茍文龍%王程%袁雪凌%王玉%彭江%盧世璧%郭全義%汪愛媛%許文靜%孟昊業%劉舒雲
서소룡%구문룡%왕정%원설릉%왕옥%팽강%로세벽%곽전의%왕애원%허문정%맹호업%류서운
唑来膦酸%成骨细胞%破骨细胞
唑來膦痠%成骨細胞%破骨細胞
서래련산%성골세포%파골세포
zoledronic acid%osteoblasts%osteoclasts
目的：观察低浓度(10-6 mol/L)唑来膦酸(zoledronate acid，ZA)对体外大鼠破骨细胞及成骨细胞的影响。方法体外分别培养大鼠来源的成骨细胞和破骨细胞，将两种细胞各分为两组：空白对照组及低浓度(10-6 mol/L)ZA组。应用抗酒石酸酸性磷酸酶染色、图像分析计算骨吸收陷窝面积，检测破骨细胞形态及骨吸收情况。碱性磷酸酶(alkaline phosphatase， ALP)染色、四甲基偶氮唑盐比色法了解成骨细胞的形态及增殖情况。结果培养1周后破骨细胞具有典型的形态特征，并在骨片上形成了吸收陷窝；ZA组与对照组相比，破骨细胞数量及生成吸收陷窝的数目和面积减少，差异有统计学意义(P＜0.05)。成骨细胞有典型的梭形、ALP染色阳性特征，培养至第7天ZA组成骨细胞光吸收值(3.37±0.11)高于对照组(2.87±0.12)，差异有统计学意义(P＜0.05)。结论低浓度(10-6 mol/L)的ZA能够抑制破骨细胞的增殖和活性，促进成骨细胞的增殖，选择恰当给药方式和剂量能够在抑制破骨的同时促进成骨。
目的：觀察低濃度(10-6 mol/L)唑來膦痠(zoledronate acid，ZA)對體外大鼠破骨細胞及成骨細胞的影響。方法體外分彆培養大鼠來源的成骨細胞和破骨細胞，將兩種細胞各分為兩組：空白對照組及低濃度(10-6 mol/L)ZA組。應用抗酒石痠痠性燐痠酶染色、圖像分析計算骨吸收陷窩麵積，檢測破骨細胞形態及骨吸收情況。堿性燐痠酶(alkaline phosphatase， ALP)染色、四甲基偶氮唑鹽比色法瞭解成骨細胞的形態及增殖情況。結果培養1週後破骨細胞具有典型的形態特徵，併在骨片上形成瞭吸收陷窩；ZA組與對照組相比，破骨細胞數量及生成吸收陷窩的數目和麵積減少，差異有統計學意義(P＜0.05)。成骨細胞有典型的梭形、ALP染色暘性特徵，培養至第7天ZA組成骨細胞光吸收值(3.37±0.11)高于對照組(2.87±0.12)，差異有統計學意義(P＜0.05)。結論低濃度(10-6 mol/L)的ZA能夠抑製破骨細胞的增殖和活性，促進成骨細胞的增殖，選擇恰噹給藥方式和劑量能夠在抑製破骨的同時促進成骨。
목적：관찰저농도(10-6 mol/L)서래련산(zoledronate acid，ZA)대체외대서파골세포급성골세포적영향。방법체외분별배양대서래원적성골세포화파골세포，장량충세포각분위량조：공백대조조급저농도(10-6 mol/L)ZA조。응용항주석산산성린산매염색、도상분석계산골흡수함와면적，검측파골세포형태급골흡수정황。감성린산매(alkaline phosphatase， ALP)염색、사갑기우담서염비색법료해성골세포적형태급증식정황。결과배양1주후파골세포구유전형적형태특정，병재골편상형성료흡수함와；ZA조여대조조상비，파골세포수량급생성흡수함와적수목화면적감소，차이유통계학의의(P＜0.05)。성골세포유전형적사형、ALP염색양성특정，배양지제7천ZA조성골세포광흡수치(3.37±0.11)고우대조조(2.87±0.12)，차이유통계학의의(P＜0.05)。결론저농도(10-6 mol/L)적ZA능구억제파골세포적증식화활성，촉진성골세포적증식，선택흡당급약방식화제량능구재억제파골적동시촉진성골。
Objective To explore the effects of low concentration (10-6 mol/L) of zoledronic acid (ZA) on osteoclasts and osteoblasts. Methods Osteoclasts and osteoblasts were obtained from neonatal rats and cultured in vitro, and they were divided into control group and ZA group. TRAP staining and image analysis were used to detect the proliferation and activity of osteoclasts. ALP staining and MTT were used to detect the morphological characteristics and proliferation of osteoblasts.Results After a week, osteoclasts showed typical morphological characteristics, and bone resorption lacunas were found in vitro. Compared with control group, the number of osteoclasts and lacunae (P<0.05), and the area of lacunae (P<0.05) in ZA group were significantly decreased. Osteoblasts showed typical fusiform and ALP showed positive staining characteristics. The optical density of osteoblasts was dramatically higher in ZA group (3.37±0.11) than control group (2.87±0.12) on day 7 after being cultured, and it had significant difference (P<0.05).Conclusion The low concentration (10-6 mol/L) of ZA can inhibit proliferation and activity of osteoclasts and promote the proliferation of osteoblasts. Therefore, it is important to choose right administration and dose of ZA to enhance the osteogenesis and inhibit the bone resorption.
