CAJ | 학술논문

目的：利用我室创建的血红蛋白释放试验，研究遗传性球形红细胞增多症患者红细胞释放血红蛋白情况。方法：采用一系列血红蛋白释放试验：单向释放电泳、双向释放电泳、全血多组分电泳和等低渗全程释放电泳（室温、37℃），比较研究患者与正常人红细胞的差异。结果：患者红细胞经单向电泳释放出的血红蛋白快泳部分缺失或明显减少。双向电泳结果，患者红细胞除快泳血红蛋白减少或缺失外，还可见红细胞及全血HbA2沿对角线分布，而且全血出现高铁血红素白蛋白。全血多组分实验中可见，患者红细胞及全血均无再释放。等低渗全程电泳显示，患者红细胞及全血的再释放都明显减弱或消失。结论：从电泳释放角度证明患者的红细胞明显异常，发现患者红细胞内快泳血红蛋白明显减少或缺失。
목적：이용아실창건적혈홍단백석방시험，연구유전성구형홍세포증다증환자홍세포석방혈홍단백정황。방법：채용일계렬혈홍단백석방시험：단향석방전영、쌍향석방전영、전혈다조분전영화등저삼전정석방전영（실온、37℃），비교연구환자여정상인홍세포적차이。결과：환자홍세포경단향전영석방출적혈홍단백쾌영부분결실혹명현감소。쌍향전영결과，환자홍세포제쾌영혈홍단백감소혹결실외，환가견홍세포급전혈HbA2연대각선분포，이차전혈출현고철혈홍소백단백。전혈다조분실험중가견，환자홍세포급전혈균무재석방。등저삼전정전영현시，환자홍세포급전혈적재석방도명현감약혹소실。결론：종전영석방각도증명환자적홍세포명현이상，발현환자홍세포내쾌영혈홍단백명현감소혹결실。
Objective:To explore the release of Hb from red blood cell (RBC) of patients with hereditary spherocytosis(HS), with the appli-cation of hemoglobin release test(HRT)established in our laboratory.Methods:To compare the difference in RBC between patients with HS and the normal individuals through a series of HRTs, including the unidimensional release electrophoresis, the two dimensional release electrophoresis, the whole blood multicomponent electrophoresis, and the isotonic and hypotonic whole journey release electrophoresis(room temperature, 37 ℃ ). Results:The fast moving Hb released from patients'RBC through the unidimensional electrophoresis was deleted or decreased obviously .The results of the two dimensional electrophoresis showed that besides that , RBC and whole blood HbA2 were by and large almost not separated from diagonal and MHA appeared in whole blood.There was no re-release from patients'RBC and whole blood in the whole blood multicomponent electrophore-sis.The isotonic and hypotonic whole journey release electrophoresis showed that the re-release was weakened obviously or disappeared from pa-tients'RBC and whole blood.Conclusion:The obvious abnormality of RBC of patients with HS is demonstrated from the angle of electrophoretic re-lease and it is found that the fast moving Hb in patients'RBC is decreased obviously or deleted.