CAJ | 학술논문

目的：检测结直肠癌组织KRAS、BRAF及PIK3CA基因突变情况，并分析其内在关系。方法：收集内蒙古巴彦淖尔市医院普外一科2011年3月至2011年8月结直肠癌手术切除标本41例，提取DNA经PCR扩增后，检测KRAS、BRAF和PIK3CA基因的突变情况，分析结直肠癌组织KRAS、BRAF及PIK3CA基因突变间的内在关系。结果：（1）15例患者的KRAS基因发生突变，突变率36．6％，5例患者的BRAF基因发生突变，突变率12．2％，7例患者的PIK3CA基因发生突变，突变率17．1％。（2）BRAF基因突变者共5例，全部为KRAS野生型的患者；PIK3CA基因突变者共7例，其中3例（42．9％）为KRAS野生型的患者，4例（57．1％）为KRAS突变型的患者；BRAF与PIK3CA基因不存在共突变。结论：（1）结直肠癌患者KRAS基因突变率较高，有必要进行常规检测。（2）KRAS野生型患者中部分患者的BRAF基因突变可能是促进结直肠癌发生发展的原因之一。 PIK3CA与KRAS基因的共突变可能促进了结直肠癌发生发展。
목적：검측결직장암조직KRAS、BRAF급PIK3CA기인돌변정황，병분석기내재관계。방법：수집내몽고파언뇨이시의원보외일과2011년3월지2011년8월결직장암수술절제표본41례，제취DNA경PCR확증후，검측KRAS、BRAF화PIK3CA기인적돌변정황，분석결직장암조직KRAS、BRAF급PIK3CA기인돌변간적내재관계。결과：（1）15례환자적KRAS기인발생돌변，돌변솔36．6％，5례환자적BRAF기인발생돌변，돌변솔12．2％，7례환자적PIK3CA기인발생돌변，돌변솔17．1％。（2）BRAF기인돌변자공5례，전부위KRAS야생형적환자；PIK3CA기인돌변자공7례，기중3례（42．9％）위KRAS야생형적환자，4례（57．1％）위KRAS돌변형적환자；BRAF여PIK3CA기인불존재공돌변。결론：（1）결직장암환자KRAS기인돌변솔교고，유필요진행상규검측。（2）KRAS야생형환자중부분환자적BRAF기인돌변가능시촉진결직장암발생발전적원인지일。 PIK3CA여KRAS기인적공돌변가능촉진료결직장암발생발전。
Objective:To detect the mutations of KRAS, BRAF and PIK3CA genes in colorectal cancer and to analyze the intrinsic relation-ship between the three genes.Method:41 samples of colorectal cancer were collected in the General Surgery Department of Banyannur Hospital from March 2011 to October 2011, whose DNAs were extracted and amplified by using polymerase chain reaction methods.The mutations of KRAS, BRAF and PIK3CA genes were detected and the intrinsic relationship between them were analyzed .Results:15, 5 and 7 patients were found mutations of KRAS, BRAF and PIK3CA genes respectively with the mutation rates being 36.6 %、12.2 % and 17.1 % respectively.(2) There were 5 patients with KRAS gene mutations, all of which were wild-type; there were 7 patients with PIK3CA mutations, 3 (42.9 %) of whom had wild-type KRAS and 4 (57.1%) of whom had KRAS mutation.There was no co -mutation between BRAF and PIK3CA genes. Conclusions: (1) KRAS gene mutation rate is high, so there is a need for regular inspection.(2) BRAF gene mutation in Some patients with wild-type BRAF gene may be one of the reasons for the occurrence and the development of colorectal cancers.The co-mutation of BRAF gene and PIK3CA gene may contribute to the occurrence and development of colorectal cancers.