CAJ | 학술논문

目的：对比两种卵巢切除术式建立大鼠骨质疏松模型的便捷性及安全性。方法40只3月龄雌性SD大鼠随机均分为2组，背侧部双切口手术组(OVX 1，n=20)、腹部正中切口手术组(OVX 2，n=20)分别进行相对应卵巢切除术，对比两种术式手术用时、出血量、术后并发症及骨密度(bone mineral density，BMD)情况。结果 OVX 1组与OVX 2组手术平均时间分别为(26.35±7.55) min和(30.95±6.39) min，OVX 1组较OVX 2组手术时间短(P＜0.05)。OVX 1组与OVX 2组平均出血量分别为(1.81±0.81) g和(1.82±0.77) g，差异无统计学意义(P＞0.05)。OVX 1组与OVX 2组术者操作成长曲线(大鼠数量-手术时间，大鼠数量-手术出血量)均呈现负性相关(r=-0.8468，P=0.000，r=-0.7882，P=0.000；r=-0.9034，P=0.000， r=-0.8912，P=0.000)。OVX 2组1例术后切口开裂感染，两组大鼠均未出现死亡情况，OVX 1组术前与术后3个月第5腰椎平均骨密度分别为(0.376±0.021) g/cm2和(0.264±0.026) g/cm2，OVX 2组为(0.371±0.016) g/cm2和(0.275±0.019) g/cm2， BMD均明显降低(P＜0.05)。3个月后两组大鼠剖腹探查发现，OVX 1组无腹腔粘连，OVX 2组腹腔粘连严重。结论背侧部双切口卵巢切除术建立大鼠骨质疏松模型操作更为简便、手术用时少、安全性高。
목적：대비량충란소절제술식건립대서골질소송모형적편첩성급안전성。방법40지3월령자성SD대서수궤균분위2조，배측부쌍절구수술조(OVX 1，n=20)、복부정중절구수술조(OVX 2，n=20)분별진행상대응란소절제술，대비량충술식수술용시、출혈량、술후병발증급골밀도(bone mineral density，BMD)정황。결과 OVX 1조여OVX 2조수술평균시간분별위(26.35±7.55) min화(30.95±6.39) min，OVX 1조교OVX 2조수술시간단(P＜0.05)。OVX 1조여OVX 2조평균출혈량분별위(1.81±0.81) g화(1.82±0.77) g，차이무통계학의의(P＞0.05)。OVX 1조여OVX 2조술자조작성장곡선(대서수량-수술시간，대서수량-수술출혈량)균정현부성상관(r=-0.8468，P=0.000，r=-0.7882，P=0.000；r=-0.9034，P=0.000， r=-0.8912，P=0.000)。OVX 2조1례술후절구개렬감염，량조대서균미출현사망정황，OVX 1조술전여술후3개월제5요추평균골밀도분별위(0.376±0.021) g/cm2화(0.264±0.026) g/cm2，OVX 2조위(0.371±0.016) g/cm2화(0.275±0.019) g/cm2， BMD균명현강저(P＜0.05)。3개월후량조대서부복탐사발현，OVX 1조무복강점련，OVX 2조복강점련엄중。결론배측부쌍절구란소절제술건립대서골질소송모형조작경위간편、수술용시소、안전성고。
Objective To compare the simplicity and safety of two operative methods in inducing osteoporosis in rats.Methods Forty 3-month old female SD rats were divided into two groups randomly: OVX 1 group (n=20) and OVX 2 group (n=20). In the first group, ovariectomy was a double dorsal-lateral incision. In the second group, ovariectomy was a midline abdominal incision. Surgical operation time, blood loss, postoperative complications and BMD were compared between two groups.ResultsThe average operation time of OVX 1 group was significantly shorter than that of OVX 2 group (26.35±7.55 minvs 30.95±6.39 min, P<0.05). The average blood loss of OVX 1 group and OVX 2group was 1.81±0.81 g and 1.82±0.77 g, respectively, with no significant difference (P>0.05). The level of surgery time and blood loss were negative correlated with the number of rats in OVX 1 and OVX 2 group (r=-0.846 8,P=0.000,r=-0.788 2,P=0.000;r=-0.903 4,P=0.000,r=-0.891 2,P=0.000). One case in OVX 2 group had complication of incision infection. No rats died in two groups. All rats hadundergone Dual X-ray Absorptiometry (DXA) Measurement before and three months after operation. The bone mineral density (BMD) of the fifth lumbar was 0.376±0.021 g/cm2 before operation and 0.264±0.026 g/cm2 three months after operation in OVX 1 group, and 0.371±0.016 g/cm2 and 0.275±0.019 g/cm2 in OVX 2 group, which were significantly reduced (P<0.05). Rats in OVX 2 group had severe abdominal cavity adhesion after three month.Conclusion The double dorsal-lateral incision of ovariectomization is technically easier, less time consuming and more safety in establishing the rat model of osteoporosis.