CAJ | 학술논문

ClC型氯离子通道蛋白质在调节生命体的新陈代谢过程中起着重要作用。在原有蛋白质ClC-ec1晶体结构的基础上，依据同源建模的原理，借助SWISS-MODEL网络服务器构建了一类重要氯离子通道蛋白质ClC-0的空间结构，同时对所得结构的合理性进行了分析。通过衡量结构的Z-score值、分析结构的空间坐标波动性以及利用视图软件VMD将建模的ClC-0结构和模板结构进行比较，发现通过同源建模所得到蛋白质ClC-0的空间结构符合后续研究的要求，这在很大程度上方便了该类蛋白质的后续计算模拟研究。
ClC형록리자통도단백질재조절생명체적신진대사과정중기착중요작용。재원유단백질ClC-ec1정체결구적기출상，의거동원건모적원리，차조SWISS-MODEL망락복무기구건료일류중요록리자통도단백질ClC-0적공간결구，동시대소득결구적합이성진행료분석。통과형량결구적Z-score치、분석결구적공간좌표파동성이급이용시도연건VMD장건모적ClC-0결구화모판결구진행비교，발현통과동원건모소득도단백질ClC-0적공간결구부합후속연구적요구，저재흔대정도상방편료해류단백질적후속계산모의연구。
CIC-type chloride ion channel proteins play important role in regulating the metabolism process of biological system. On the base of CIC-ec1 crystal structure,in accordance with the principle of homology modeling,using SWISS-MODEL net server,constructed the spatial structure of a kind of CIC-0 chloride ion channel protein , and analyzed the reasonableness of the obtained structure. Through measuring the Z-score of the structure ,analyzing the fluctuation of space coordinates of the structure , and comparing the modeling CIC-0 structure with template structure,discovered that the spatial structure of CIC-0 protein by homology modeling complied with the requirement of further study ,which makes the further simulation and computation be easy.